| 骨髓内皮细胞条件培养液对巨核系细胞体外增殖的影响 |
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| 引用本文： | 黄艳红,王绮如. 骨髓内皮细胞条件培养液对巨核系细胞体外增殖的影响[J]. 中南大学学报(医学版), 2004, 29(4): 386-388 |
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| 作者姓名： | 黄艳红 王绮如 |
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| 作者单位： | 中南大学湘雅医学院血液生理研究室,长沙,410078;中南大学湘雅医学院血液生理研究室,长沙,410078;中南大学生殖与干细胞工程研究所,长沙,410078 |
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| 基金项目： | 国家自然科学基金资助 (39970 0 92，30 0 30 0 70 ) |
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| 摘 要： | 目的: 探讨骨髓内皮细胞条件培养液（E-CM）对巨核系细胞体外增殖的调节作用。方法: 分离纯化骨髓内皮细胞和骨髓成纤维细胞，分别收集其无血清条件培养液，比较两种无血清条件培养液对体外扩增成熟巨核细胞和巨核系祖细胞的作用。结果: E-CM和骨髓成纤维细胞条件培养液（F-CM）对成熟巨核细胞及巨核系祖细胞有体外扩增作用；E-CM对巨核系祖细胞的扩增作用明显优于F-CM（P<0.05）；对成熟巨核细胞的扩增作用弱于F-CM（P<0.01）。结论: E-CM对巨核系细胞, 主要是巨核系祖细胞有体外扩增作用。
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| 关 键 词： | 骨髓内皮细胞 条件培养液 巨核细胞 体外扩增 |
| 文章编号： | 1672-7347(2004)04-0386-03 |
| 修稿时间： | 2004-04-19 |
Effects of bone marrow endothelial cell conditioned medium on the expansion of mature megakaryocytes and CFU-Meg in vitro |
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| HUANG Yan hong ,WANG Qi ru ,. Effects of bone marrow endothelial cell conditioned medium on the expansion of mature megakaryocytes and CFU-Meg in vitro[J]. Journal of Central South University. Medical sciences, 2004, 29(4): 386-388 |
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| Authors: | HUANG Yan hong WANG Qi ru |
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| Affiliation: | 1.Research Laboratory of Blood Physiology, Xiangya School of Medicine，Central South University,Changsha 410078;
2.Reproduction and Stem Cell Engineering Institute,Central South University，Changsha 410078，China |
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| Abstract: | Objective To investigate the effects of serum free bone marrow endothelial cell conditioned medium (E CM) and serum free bone marrow fibroblast conditioned medium (F CM) on the expansion of mature megakaryocytes and CFU Meg . Methods E CM and F CM were collected and added in liquid culture system respectively. Results E CM and F CM significantly promoted the expansion of mature megakaryocytes and CFU Meg in liquid culture system. The effect of E CM on the expansion of CFU Meg was better than the effect of F CM, While the effect of E CM on the expansion of mature megakaryocytes was inferior to the effect of F CM.Conclusion E CM can be used to expand CFU Meg while F CM can be used to expand the mature megakaryocytes in vitro. |
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| Keywords: | bone marrow endothelial cell conditioned medium megakaryocytes expansion in vitro |
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