染色体STR遗传标记在亲缘鉴定中的应用

目的：探讨染色体STR遗传标记在亲缘关系鉴定中的应用。方法：用Profiler plus及Cofiler试剂盒检测468例亲缘鉴定案，对其中STR基因座不符合遗传规律者，增加HLA等血型基因和“PowerPlex16TM体系试剂检测。必要时还增加Y-STR，X-STR基因座检测和HLA等位基因测序。结果：408例亲缘个体的亲权概率W>0.95，其中350例亲缘个体的W>0.9995；60例无关个体的W<0.8，其中48例W<0.27。经χ2检验，有亲缘关系的个体和无亲缘关系的个体间全相同和全不同的基因座数差异均有统计学意义（P<0.001）；半相同的基因座数差异无统计学意义（P>0.05）。结论：染色体STR遗传标记用于鉴定亲缘关系时，当两个体不同基因座个数≤1个，或全相同基因座数≥6个时，属亲缘关系。

Chromosome STR genetic markers in paternity identification

Objective To explore the application of paternity identification by chromosome STR genetic markers (short tandem repeat). Methods The paternity testings in 468 cases were routinely carried out using amphibian STR Profiler plus and Cofiler PCR amplification kits. When STR exclusions were found, HLA system and other blood groups were detected by molecular typing and loci of PowerPlex 16 TM system. STR loci were genotyped. If necessary, the genotyping of Y chromosome specific STR,X chromosome specific STR and HLA allelic sequencing were added.Results W of 408 cases of paternity was more than 0.95, and that of 350 cases was more than 0.9995. W of 60 cases of unrelated individuals was less than 0.8, and that of another 48 cases was less than 0.27. By Chi Square test,there was significant difference ( P <0.001) in the entire same allelic genes and the entire different allelic genes and no significant difference ( P >0.05) in the half same between allelic genes the two groups.Conclusion It is effective to identify the paternity by STR genetic markers. The pair is related when the locus number of entirely different allelic genes is less than 1 or that of the entirely same allelic genes is more than 6.