| 抗黄曲霉毒素B_1蛋黄抗体的制备及其性质的研究 |
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| 引用本文： | 陈福生,李根久,陈九武,罗信昌,周启.抗黄曲霉毒素B_1蛋黄抗体的制备及其性质的研究[J].卫生研究,1999(4). |
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| 作者姓名： | 陈福生 李根久 陈九武 罗信昌 周启 |
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| 作者单位： | 华中农业大学农业微生物重点实验室，湖北省农业科学院 |
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| 基金项目： | 湖北省科学技术委员会重点课题 |
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| 摘 要： | 将黄曲霉毒Ｂ１－牛血清白蛋白（ＡＦＢ１Ｏ—ＢＳＡ）连接物免疫注射４只（Ａ、Ｂ、Ｃ、Ｄ）产蛋鸡。研究了抗ＡＦＢ１蛋黄抗体的产生进程，除鸡Ｂ外，其它３只鸡从第一次注射抗原后的第９０天开始有较明显的抗体产生，第１３５天达到高峰，从第１６５天开始下降。在抗体产生高峰期，蛋黄抗体Ａ、Ｃ、Ｄ的间接ＥＬＩＳＡ效价分别为１∶８０００、１∶６０００、１∶６０００。以蛋黄抗体Ａ为材料，研究了抗体的特异性，结果表明：该抗体有较好的特异性。但是抗体与ＡＦＢ１的结构类似物（ＡＦＢ２、ＡＦＧ２、ＡＦＧ１、ＡＦＭ１）除ＡＦＭ１外都有不同程度的交叉反应，达到５０％的竞争抑制率时，ＡＦＢ１、ＡＦＢ２、ＡＦＧ１、ＡＦＧ２的灵敏度分别为６、２５、１２５和２４９５ｎｇ／ｍｌ。
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| 关 键 词： | 黄曲霉毒素B_1 抗体 产蛋鸡 酶联免疫分析法 |
Studies on the preparation and characters of anti AFB 1 antibody from the yolk of laying hens |
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| Chen Fusheng Li Genjiu,Chen Jiuwu,Luo Xingchang,Zhou Qi Agro Microbiology Key Laboratories Huazhong Agricultural University,Wuhan ,China.Studies on the preparation and characters of anti AFB 1 antibody from the yolk of laying hens[J].Journal of Hygiene Research,1999(4). |
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| Authors: | Chen Fusheng Li Genjiu Chen Jiuwu Luo Xingchang Zhou Qi Agro Microbiology Key Laboratories Huazhong Agricultural University Wuhan China |
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| Institution: | Chen Fusheng Li Genjiu,Chen Jiuwu,Luo Xingchang,Zhou Qi Agro Microbiology Key Laboratories Huazhong Agricultural University,Wuhan 430070,China |
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| Abstract: | AFB 1O BSA conjugates were injected into four laying hens(A,B,C and D).The yolk anti AFB 1 antibodies A,C and D produced were obviously increased after 90 days of the first injection,and reached the peak after 135 days,and started dropping after 165 days.The indirect ELISA titer of yolk antibodies A,C and D were 1∶8000,1∶6000 and 1∶6000 respectively.The specificity of the antibody A was good, but a cross reaction with AFB 2,AFG 1,AFG 2, with an exception of AFM 1,was found.The sensitivities of AFB 1,AFB 2,AFG 1 and AFG 2 were 6,25,125 and 2495 μg/L respectively. |
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| Keywords: | aflatoxin B 1 antibody laying hen ELISA |
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