Antitumor effects of the fibroblasts transfected TNF-α gene and its mutants |
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Authors: | Li Qingfen Li Li Li Zhuoya Gong Feili Feng Wei Jiang Xiaodan Xiong Ping |
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Institution: | (1) Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan |
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Abstract: | Summary To compare the anti-tumor effects of transmembrane TNF-α (TM-TNF) and secreted TNF-α (S-TNF)in vivo, mouse fibroblasts NIH3T3 were transfected separately with three types of retrovirus containing wild type TNF-α (Wt-TNF),
TM-TNF mutant (TM-TNFm), S-TNF mutant (S-TNFm). Southern blot, RT-PCR, FACS and bioassay were used to investigate TNF-α gene
integration, expression and its biological activity. It was found that both fixed cells and supernatant of NIH3T3/Wt-TNF,
the fixed cells of NIH3T3/TM-TNFm and the supernatant of NIH3T3/S-TNFm could express high level of TNF-α or its mutants and
effectively kill H22in vitro. The trans-fected NIH3T3 were separately injected into the mice at the sites of H22 tumor cell inoculation according to a
ratio of 5∶1 or 1∶1 (effector/target cells, E/T) after the third day of H22 challenge, respectively. At the E/T=5∶1, the NIH3T3/TM-TNFm
induced the highest tumor regression, while NIH3T3/S-TNFm exerted the strongest tumor depressing effect at the E/T=1∶1in vivo. No obvious side effects were noted throughout the course of treatment. The results suggest that both TM-TNF and S-TNF could
cause tumor regression. The anti-tumor effect of TM-TNF would be more powerful and safe than that of S-TNF at the proper E/T
ratio.
LI Qingfen, female, born in 1965, Associate Professor |
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Keywords: | tumor gene therapy transmembrane TNF-α retroviral vector |
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