Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation |
| |
Authors: | Feng Xue;Er-jun Wu;Pei-xun Zhang;Li-ya A;Yu-hui Kou;Xiao-feng Yin;Na Han; |
| |
Institution: | Feng Xue;Er-jun Wu;Pei-xun Zhang;Li-ya A;Yu-hui Kou;Xiao-feng Yin;Na Han;Department of Trauma and Orthopedics,Peking University People’s Hospital;Graduate School of Shanxi Medical University;Central Laboratory,Peking University People’s Hospital; |
| |
Abstract: | We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staining revealed that biological conduit sleeve bridging reduced glial scar formation and spinal muscular atrophy after spinal cord hemisection. Bone marrow mesenchymal stem cells survived and proliferated after transplantation in vivo, and differentiated into cells double-positive for S100(Schwann cell marker) and glial fibrillary acidic protein(glial cell marker) at 8 weeks. Retrograde tracing showed that more nerve fibers had grown through the injured spinal cord at 14 weeks after combination therapy than either treatment alone. Our findings indicate that a biological conduit combined with bone marrow mesenchymal stem cell transplantation effectively prevented scar formation and provided a favorable local microenvironment for the proliferation, migration and differentiation of bone marrow mesenchymal stem cells in the spinal cord, thus promoting restoration following spinal cord hemisection injury. |
| |
Keywords: | nerve regeneration spinal cord injury spinal cord hemisection biological conduit bone marrow mesenchymal stem cells stem cells transmission electron microscope cell transplantation neurons nerve fibers NSFC grants neural regeneration |
本文献已被 CNKI 等数据库收录! |