参芎化瘀胶囊预处理对大鼠脑缺血再灌注损伤后一氧化氮合酶亚型表达的影响

目的：观察参芎化瘀胶囊预处理对大鼠脑缺血再灌注损伤后一氧化氮合酶（NOS）亚型内皮型（eNOS）、神经元型（nNOS）及诱导型（iNOS）表达的影响，探讨参芎化瘀胶囊对大鼠急性脑缺血再灌注损伤保护作用的机制。方法: 实验大鼠随机分为：假手术组，缺血再灌注组，参芎化瘀胶囊高、中、低剂量预处理组（480、240、120 mg?kg－1），各组又分为缺血2 h 再灌注后3、6、12、24、48、72 h 组，每组6 只。灌胃给药7天，每天1 次，第7 天灌胃2 h 后线栓法制作大脑中动脉阻断（MCAO）再灌注模型。免疫组化方法检测eNOS、nNOS 和iNOS 蛋白表达。结果：①与假手术组比较，缺血再灌注组各时间点（3、6、12、24、48、72 h）eNOS、nNOS 和iNOS 表达均增强（P<0.05 或P<0.01）；于与缺血再灌注组比较，参芎化瘀胶囊预处理组各时间点eNOS 表达均增强（P<0.05 或P<0.01），nNOS 和iNOS 表达均减少（P<0.05 或P<0.01），其中均以高剂量组效果最为显著。结论：参芎化瘀胶囊预处理对脑缺血再灌注损伤具有保护作用，其作用机制可能与调节NOS 分型表达有关。

Regulating Effects of Shen-Xiong Hua-Yu Capsule Preconditioning on Expression Subtype of NOS in Cerebral Ischemia-reperfusion Injury among Rats

This study was aimed to observe the regulating effect of Shen-Xiong Hua-Yu (SXHY) capsule preconditioning on the expression of subtypes of nitric oxide synthase (NOS), including endothelial nitric oxide synthase(eNOS), nervous nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), in cerebral ischemia-reperfusion injury (CIRI) among rats, and to further clarify the mechanism of protective effect by SXHY capsule on acute CIRI rats. Rats were randomly divided into the sham operation group, CIRI group, and SXHY capsule of high-,medium-, and low-dose preconditioning group (480, 240, 120 mg·kg-1). Each group was further randomly dividedinto different subgroups, which were the 3, 6, 12, 24, 48, 72 h group after 2 h CIRI (n = 6). Intragastric administration was given once a day for 7 days. The middle cerebral artery occlusion (MCAO) and reperfusion model was reproduced by an intraluminal filament method on the 7th day. The protein expressions of eNOS, nNOS and iNOS were measured by immunhistochemical method. The results showed that compared with the sham operation group, expressions of eNOS, nNOS and iNOS in the CIRI group were increased at different time points (i.e., 3, 6, 12, 24, 48, 72 h, P < 0.05 or P < 0.01). Compared with the CIRI group, eNOS expression increased at different time points in SXHY capsule group (P < 0.05 or P < 0.01). The nNOS and iNOS expression decreased at different time points (P <0.05 or P < 0.01). Among them, the high-dose group was the group with the most obvious effect. It was concludedthat SXHY capsule preconditioning had protective effect on CIRI. Its mechanism may be related to the regulation on protein expression of NOS subtypes.