甲氧滴滴涕对雄性生殖系统的氧化损伤作用

目的观察甲氧滴滴涕(MXC)对雄性小鼠的生殖系统毒性作用并探讨其毒性作用的可能机制.方法光镜观察喂服MXC小鼠睾丸和附睾的变化,并以生物化学的方法测定氧化损伤相关指标的改变.结果精子活动度和活率降低,畸形率增高,生精小管上皮变薄;睾丸和附睾的谷胱甘肽过氧化物酶(GSH-Px)活力和超氧化物歧化酶(SOD)活力显著下降(p＜0.001),丙二醛(MDA)含量明显升高(P＜0.01),维生素E组各项指标的变化均较21 d组的变化小(P＜0.05).结论MXC对雄性小鼠生殖系统具有一定的毒性作用,可导致睾丸和附睾抗氧化能力下降,脂质过氧化增加,其毒性作用可被维生素E减轻.

Oxidative damage of methoxychlor on male reproductive system

Objective The aim of this study is to observe the toxic effect of MXC on genital system of male mice and to explore the possible toxic mechanism. [Methods] We observed the diversity of testis and epididymis of mice fed with MXC under microscope, and detected some indexes related to oxidative damage by using biochemistry methods. [Results] In the epididymis, the sperm activity and alive proportion rate decreased significantly, and the rate of malformation was raised. The spermatogenic epithelium was thinner than the controlled. The activities of GSH-PX and SOD in three MXC groups decreased significantly than the control group (P <0.001), but the quantities of MDA were raised in comparison to the control group (P<0.01). In vitamin E group the change degree of each parameter was lighter than the group exposed to MXC 21 days(P<0.05). [Conclusions] MXC can act venomous effectiveness on reproductive system of male mice. MXC can decrease the capability of anti-oxidizing in testis and epididymis, and increase lipid peroxidation.VitE can weaken the effect of MXC.