| JNK在棕榈酸致骨骼肌细胞胰岛素抵抗中的作用 |
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| 引用本文： | 高静,王慧,牛文彦.JNK在棕榈酸致骨骼肌细胞胰岛素抵抗中的作用[J].天津医药,2011,39(1):53-55. |
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| 作者姓名： | 高静 王慧 牛文彦 |
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| 作者单位： | 天津医科大学免疫教研室,300070 |
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| 基金项目： | 国家自然科学基金资助项目，国家自然科学基金委员会-加拿大卫生研究院健康研究合作计划项目，天津市科委科技支撑计划项目 |
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| 摘 要： | 目的:探讨棕榈酸造成L6GLUT4myc骨骼肌细胞胰岛素抵抗过程中c-JunN-末端激酶(JNK)所起的作用。方法:将L6GLUT4myc成肌细胞培养于24孔和6孔培养板中,随机分为溶剂组和棕榈酸组,分别用0.3mmol/L的棕榈酸盐和溶剂牛血清白蛋白(BSA)孵育16h,在棕榈酸组的后30min加入JNK的抑制剂,于胰岛素刺激前后用酶联免疫吸附测定(ELISA)细胞膜上GLUT4myc的含量,免疫印迹法测定蛋白激酶B(Akt)、JNK和胰岛素受体底物1(IRS1)的磷酸化。结果:与溶剂组相比,棕榈酸组中胰岛素增加的GLUT4myc水平的倍数和Akt的磷酸化降低(P<0.05);JNK和IRS1的丝氨酸位点S307的磷酸化水平变化差异无统计学意义(P>0.05)。结论:棕榈酸导致L6骨骼肌细胞胰岛素抵抗的机制可能不涉及JNK,或JNK的作用很小。
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| 关 键 词： | 棕榈酸 肌 骨骼 葡萄糖转运体4型 胰岛素 胰岛素抗药性 JNK丝裂原活化蛋白激酶类 蛋白激酶类 |
The Role of JNK in the PaImitate-Induced Insulin Resistance in Skeletal Muscle Cells |
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| GAO Jing,WANG Hui,NIU Wenyan.The Role of JNK in the PaImitate-Induced Insulin Resistance in Skeletal Muscle Cells[J].Tianjin Medical Journal,2011,39(1):53-55. |
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| Authors: | GAO Jing WANG Hui NIU Wenyan |
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| Institution: | GAO Jing,WANG Hui,NIU Wenyan Department of Immunology,Tianjin Medical University,Tianjin 300070,China |
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| Abstract: | Objective: To study the role of c-jun N-terminal kinase (JNK) in the mechanism of palmitate-induced insulin resistance in L6GLUT4myc skeletal muscle cells. Methods: L6GLUT4myc myoblasts were incubated in 24 and 6 wells plates and divided into two groups, and treated with 0.3 mmol / L palmitate or solvent BSA for 16 h, respectively. JNK inhibitor was added to the medium during the last 30 min incubation with palmitate or BSA. The amount of GLUT4myc on the cell surface was measured by enzyme linked immunosorb... |
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| Keywords: | palmitic acid muscle skeletal glucose transporter type 4 insulin insulin resistance JNK mitogen-activated protein kinases protein kinases |
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