| 差速贴壁法培养大鼠骨髓内皮祖细胞的实验研究 |
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| 引用本文： | 李辰运,陈剑秋,孙晋津,张晓宇. 差速贴壁法培养大鼠骨髓内皮祖细胞的实验研究[J]. 天津医药, 2011, 39(4): 353-356. DOI: 10.3969/j.issn.0253-9896.2011.04.023 |
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| 作者姓名： | 李辰运 陈剑秋 孙晋津 张晓宇 |
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| 作者单位： | 天津医科大学第二医院外科,300211 |
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| 基金项目： | 天津市自然科学基金资助项目 |
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| 摘 要： | 目的:探索一种经济、可靠、稳定、高纯度的内皮祖细胞培养方法。方法:采用密度梯度离心法分离培养大鼠骨髓血管内皮祖细胞,于包被人纤连蛋白的25mL培养瓶中贴壁培养,收集48h后的未贴壁细胞培养至第7天,通过细胞摄取DiL-acLDL、结合FITC-UEA-1的荧光双染阳性率从功能角度鉴定细胞,并通过流式细胞术动态测定细胞表面抗原CD133、flk-1、VE-cadherin(CD144)及CD31进一步鉴定。结果:48h未贴壁细胞培养,细胞呈短梭形、多角形,胞体小;可见到大量典型的内皮祖细胞克隆;细胞摄取DiL-acLDL,结合FITC-UEA-1荧光双染阳性率为(87.0±6.0)%。细胞表面抗原CD133、flk-1、CD144和CD31阳性率分别为61.01%、8.55%、6.18%和8.20%。结论:差速贴壁法是一种经济、可靠、稳定、高纯度的内皮祖细胞培养方法。
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| 关 键 词： | 骨髓细胞 细胞分离 细胞,培养的 单核细胞 内皮细胞 大鼠,Wistar |
Endothelial Progenitor Cells Derived from Rat Bone Marrow Culture with Differential Adhesion Method |
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| LI Chenyun,CHEN Jianqiu,SUN Jinjin,ZHANG Xiaoyu. Endothelial Progenitor Cells Derived from Rat Bone Marrow Culture with Differential Adhesion Method[J]. Tianjin Medical Journal, 2011, 39(4): 353-356. DOI: 10.3969/j.issn.0253-9896.2011.04.023 |
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| Authors: | LI Chenyun CHEN Jianqiu SUN Jinjin ZHANG Xiaoyu |
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| Affiliation: | LI Chenyun,CHEN Jianqiu,SUN Jinjin,ZHANG Xiaoyu Second Hospital of Tianjin Medical University,Tianjin 300211,China |
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| Abstract: | Objective:To explore an economic, reliable, stable and high purity of endothelial progenitor cells(EPCs) in culture methods. Methods:Rat bone marrow-derived EPCs were separated by density gradient centrifugation and cultured,which were then plated on 25 mL culture flask adherent culture with human fibronection. The no-adherent cells cultured for 48 h were collected and cultured for 7 days, and then identified by being observed the experiment of uptaking DiL-acLDL and combining FITC-UEA-1 by fluorescence mic... |
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| Keywords: | bone marrow cells cell separation cells cultured monocytes endothelial cells rats Wistar |
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