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| 闭塞性周围动脉粥样硬化患者血管内皮损伤的研究 | |
| 引用本文： | 门剑龙,李刚,任静. 闭塞性周围动脉粥样硬化患者血管内皮损伤的研究[J]. 天津医药, 2011, 39(3): 215-217. DOI: 10.3969/j.issn.0253-9896.2011.03.008 |
| 作者姓名： | 门剑龙 李刚 任静 |
| 作者单位： | 1. 300072,天津大学,天津市生物医学检测技术与仪器重点实验室;天津医科大学总医院临床检验科 2. 天津市生物医学检测技术与仪器重点实验室,天津大学,300072 3. 天津医科大学总医院临床检验科 |
| 摘 要： | 目的:观察闭塞性周围动脉粥样硬化(ASO)患者的凝血和纤溶系统功能的变化,探讨ASO患者的内皮损伤机制。方法:选择ASO患者82例(患者组)和健康对照80例(对照组),采用流式细胞仪测定循环内皮细胞(CEC)和内皮祖细胞(EPCs)数量。采用酶联免疫吸附法测定凝血酶-抗凝血酶复合物(TAT)。采用血液凝固仪测定凝血因子Ⅶ活性(FⅦ:A)、纤维蛋白原(FIB)、vW因子抗原水平(vWF∶Ag)、组织型纤溶酶原激活物活性(t-PA∶A)及纤溶酶原活化抑制物活性(PAI∶A)水平。以vWF∶Ag参考范围上限(150%)作为医学决定水平,将患者组分为vWF∶Ag正常组(38例)和vWF∶Ag增高组(44例)。结果:患者组PAI∶A、FⅦ∶A、FIB、vWF∶Ag、TAT和CEC高于对照组,EPCs低于对照组,vWF∶Ag增高组的PAI∶A、FⅦ∶A和TAT均高于vWF∶Ag正常组,差异有统计学意义(均P<0.01);患者组vWF∶Ag与FⅦ∶A、PAI∶A、TAT均呈正相关(P<0.01)。结论:ASO患者存在较严重的血管内皮损伤,内皮细胞大量脱落,且损伤后的修复能力不足,其纤溶和凝血系统功能紊乱和血管内皮损伤程度相关。 |
| 关 键 词： | 动脉粥样硬化 内皮细胞 内皮,血管 血液凝固因子 纤溶酶 流式细胞术 |
Study on Endothelial Cells Damage in Patients with Peripheral Arteriosclerosis Obliterans |
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| MEN Jianlong,LI Gang,REN Jing. Study on Endothelial Cells Damage in Patients with Peripheral Arteriosclerosis Obliterans[J]. Tianjin Medical Journal, 2011, 39(3): 215-217. DOI: 10.3969/j.issn.0253-9896.2011.03.008 | |
| Authors: | MEN Jianlong LI Gang REN Jing |
| Affiliation: | MEN Jianlong,LI Gang,REN Jing Tianjin Key Laboratory of Biomedical Detection & Instruments,Tianjin University,Tianjin 300072,China |
| Abstract: | Objective:To observe the changes of function for the coagulation and fibrinolysis system in patients with peripheral arteriosclerosis obliterans (ASO), and the mechanism of the endothelial cell damage thereof. Methods:Eighty-two ASO patients and 80 controls were selected from our hospital. The flow cytometry was used to measure the circulating endothelial cell (CEC) and the endothelial progenitor cells (EPCs). Thrombin-antithrombin complex (TAT) was measured by ELISA. Using the blood coagulation instrument ... |
| Keywords: | atherosclerosis endothelial cells endothelium vascular blood coagulation factors plasmin flow cytometry |
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