| BRMS1对卵巢癌细胞c13*转移侵袭的影响及机制 |
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| 引用本文： | 饶玉梅,方勇,韩志强,卢运萍.BRMS1对卵巢癌细胞c13*转移侵袭的影响及机制[J].华中科技大学学报(医学版),2011,40(2):131-136. |
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| 作者姓名： | 饶玉梅 方勇 韩志强 卢运萍 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院妇产科,武汉,430030 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金 |
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| 摘 要： | 目的探讨乳腺癌转移抑制基因1(BRMS1)对c13*细胞转移侵袭、粘附的影响及机制。方法由质粒PC-MV-HA-BRMS1中扩增目的基因BRMS1,将其插入pcDNA3.1(+)质粒中,构建pcDNA3.1(+)-BRMS1质粒。经酶切及测序鉴定正确后,转染c13*细胞,PCR、Real-time PCR、Western blot检测BRMS1的表达;划痕实验、Transwell实验、粘附实验检测c13*细胞运动、侵袭、粘附能力;Western blot检测整合素β1、p-AKT、AKT表达。结果成功构建了pcDNA3.1(+)-BRMS1质粒;转染目的基因的c13*细胞中,BRMS1的mRNA和蛋白表达均显著增加,细胞运动、侵袭、粘附能力降低,整合素β1及p-AKT表达均下降,AKT表达无改变。结论成功构建pcDNA3.1(+)-BRMS1质粒。BRMS1可能通过抑制整合素β1/AKT通路,抑制卵巢癌c13*细胞的运动、侵袭和粘附能力。
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| 关 键 词： | 乳腺癌转移抑制基因1 整合素β1 卵巢癌 肿瘤侵袭 肿瘤转移 |
Effect of BRMS1 on Migration,Invasion and Adhesion in Ovarian Cancer Cells(c13)and Mechanisms in vitro |
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| Rao Yumei,Fang Yong,Han Zhiqiang et al.Effect of BRMS1 on Migration,Invasion and Adhesion in Ovarian Cancer Cells(c13)and Mechanisms in vitro[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(2):131-136. |
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| Authors: | Rao Yumei Fang Yong Han Zhiqiang |
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| Institution: | Rao Yumei,Fang Yong,Han Zhiqiang et al Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To construct and express the recombinant plasmid of pcDNA3.1(+)-BRMS1 in ovarian cancer cells(c13*),and observe the effects of BRMS1 on migration,invasion and adhesion of c13* cells.Methods BRMS1 cDNA was obtained from the plasmid PCMV-HA-BRMS1 by PCR,and inserted into pcDNA3.1(+)plasmid to generate a recombinant plasmid pcDNA3.1(+)-BRMS1.The recombinant plasmid was verified by sequencing and enzyme digestion analysis,and transfected into c13* cells by using liposome.The BRMS1 expression was detec... |
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| Keywords: | breast cancer metastasis suppressor 1 integrin beta 1 ovarian cancer invasion migration |
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