| 骨髓间充质干细胞促进大鼠80%肝切除后残肝再生及修复作用的研究 |
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| 引用本文： | 龚慧宾,沈中阳,宋红丽,郑卫萍,董冲,宋晓静,张静.骨髓间充质干细胞促进大鼠80%肝切除后残肝再生及修复作用的研究[J].天津医药,2011,39(2):149-152,194. |
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| 作者姓名： | 龚慧宾 沈中阳 宋红丽 郑卫萍 董冲 宋晓静 张静 |
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| 作者单位： | 1. 天津医科大学一中心临床学院,300192
2. 天津市第一中心医院器官移植中心 |
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| 基金项目： | 天津市应用基础与前沿技术研究计划资助项目 |
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| 摘 要： | 目的:观察骨髓间充质干细胞(BMSCs)对大鼠自体80%肝切除后残余肝脏再生及修复的作用。方法:采用密度梯度离心联合贴壁筛选法分离纯化鉴定BMSCs,取6～8周体质量200～220g的大鼠,建立大鼠80%肝切除模型,实验组16只,术后即刻经门静脉注射BMSCs;对照组20只,注射等量生理盐水。采用生物化学方法检测肝功能,流式细胞仪和免疫组化检测肝细胞周期和增殖细胞核抗原(PCNA)。结果:体外成功分离培养、扩增BMSCs;实验组术后生存15只,死亡1只,对照组生存15只,死亡5只;肝脏组织显示,实验组术后1、3和7d时肝窦轻度扩张,较少的炎性细胞浸润,少见坏死,坏死程度明显轻于对照组,术后3d差异尤为明显;实验组术后1、3和7d时血ALT和AST水平较对照组明显降低,差异有统计学意义(P<0.05);实验组术后1、3和7d时肝细胞周期中S期细胞比例与对照组相比均明显增高(P<0.05);残肝免疫组化显示实验组PCNA表达水平明显高于对照组(P<0.05)。结论:BMSCs可以促进大鼠80%肝切除后残余肝脏细胞的再生及修复。
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| 关 键 词： | 骨髓 间质干细胞移植 肝切除术 肝再生 细胞周期 增殖细胞核抗原 大鼠 Wistar |
Effects of Bone Marrow Mesenchymal Stem Cells on Liver Regeneration and Repair after 80% Hepatectomy in Rats |
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| GONG Huibin,SHEN Zhongyang,SONG Hongli,ZHENG Weiping,DONG Chong,SONG Xiaojing,ZHANG Jing.Effects of Bone Marrow Mesenchymal Stem Cells on Liver Regeneration and Repair after 80% Hepatectomy in Rats[J].Tianjin Medical Journal,2011,39(2):149-152,194. |
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| Authors: | GONG Huibin SHEN Zhongyang SONG Hongli ZHENG Weiping DONG Chong SONG Xiaojing ZHANG Jing |
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| Institution: | GONG Huibin,SHEN Zhongyang,SONG Hongli,ZHENG Weiping,DONG Chong,SONG Xiaojing,ZHANG JingDepartment of Organ Transplantation,The First Central Clinic College of Tianjin Medical University,Tianjin 300192,China |
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| Abstract: | Objective: To investigate the effects of rat bone marrow mesenchymal stem cells (BMSCs) on liver regeneration and repair after 80% hepatectomy.Methods: BMSCs were isolated and purified by density gradient centrifugation combined adherent method.The model of 80% hepatectomy was established with rats aged 6-8 weeks and weighted 200~220 g.Experimental group contained 16 rats with BMSC injection via the portal veins,and control group included 20 rats with saline injection by the same way.The liver functions wer... |
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| Keywords: | bone marrow mesenchymal stem cell transplantation hepatectomy liver regeneration cell cycle proliferation cell nuclear antigen rats Wistar |
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