| 阿片生长因子抑制乳腺癌细胞生长的研究 |
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| 引用本文： | 邢恩鸿,胡建功,王宁,赵国军,邵雪斋.阿片生长因子抑制乳腺癌细胞生长的研究[J].天津医药,2011,39(1):10-13,98. |
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| 作者姓名： | 邢恩鸿 胡建功 王宁 赵国军 邵雪斋 |
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| 作者单位： | 1. 河北省承德医学院附属医院中心实验室,067000
2. 河北省承德医学院附属医院病理科,067000 |
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| 基金项目： | 河北省科学技术研究与发展计划项目 |
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| 摘 要： | 目的:探讨阿片生长因子(OGF)对MCF-7人乳腺癌细胞生长的影响。方法:细胞计数观察OGF、阿片生长因子受体(OGFr)拮抗剂纳洛酮(Naloxone)组及对照组细胞数的差别;MTT实验观察应用OGF、Naloxone及OGF联合Naloxone(OGF+Naloxone)细胞增殖抑制率的变化;流式细胞术(FCM)细胞周期分析OGF、Naloxone对MCF-7细胞生长的影响。结果:细胞计数显示,OGF处理组细胞数明显低于Naloxone处理组及对照组(P<0.01),而后2组细胞数差异无统计学意义(P>0.05);MTT实验显示,OGF处理组细胞增殖抑制率明显高于Naloxone处理组及OGF+Naloxone处理组(P<0.01),而后2组差异无统计学意义(P>0.05);OGF处理组MCF-7细胞G0/G1期分数明显高于Naloxone处理组及对照组(P<0.01),而后2组差异无统计学意义(P>0.05)。结论:OGF能抑制MCF-7人乳腺癌细胞的生长。OGFr拮抗剂能阻断这种作用。OGF阻断细胞周期于G0/G1期。
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| 关 键 词： | 阿片 受体 生长因子 乳腺肿瘤 细胞系 肿瘤 细胞周期 纳洛酮 |
Inhibitory Effect of Opioid Growth Factor on Human Breast Carcinoma Cells |
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| XING Enhong,HU Jiangong,WANG Ning,ZHAO Guojun,SHAO Xuezhai.Inhibitory Effect of Opioid Growth Factor on Human Breast Carcinoma Cells[J].Tianjin Medical Journal,2011,39(1):10-13,98. |
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| Authors: | XING Enhong HU Jiangong WANG Ning ZHAO Guojun SHAO Xuezhai |
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| Institution: | XING Enhong,HU Jiangong,WANG Ning,ZHAO Guojun,SHAO Xuezhai Central laboratory,Affiliated Hospital of Chengde Medecal College,Chengde 067000,China |
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| Abstract: | Objective:To investigate the inhibitory effect of opioid growth factor (OGF)on MCF-7 human breast carcinoma cells. Methods:The difference in cell counting was compared between OGF group and the opioid antagonist naloxone group. The MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay was used to detect the inhibiting rate of cell growth in OGF, naloxone and OGF combined naloxone(OGF+naloxone)groups. And flow cytometry was used to detect the effects of OGF and naloxone on the cell cycle d... |
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| Keywords: | opium receptors growth factor breast neoplasms cell line tumor cell cycle NALOXONE |
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