抗核抗体和ENA谱联合检测的临床应用价值

目的探讨抗核抗体(ANA)和ENA谱在就诊患者中的阳性分布趋势。方法 ANA检测采用间接免疫荧光法,ENA谱检测采用斑点法,采用SPSS11.0进行数据统计分析。结果 3332例患者的ANA阳性率为33.67%,其中男性为19.74%,女性为38.69%,二者差异有统计学意义(P<0.01)。不同年龄组间ANA阳性患者的阳性率也有差异(≤20岁组28.2%、21~49岁组36.4%、≥50岁组29.6%,P<0.01)。ANA阳性患者的荧光模式分析,以核颗粒型28.8%、核均质型20.3%、胞浆颗粒型11%和核均质/核颗粒型10.6%居多。ANA滴度≥1∶100男性占20.71%,女性占79.29%;≥1∶320男性占14.78%,女性占85.22%;≥1∶1000男性占10.94%,女性占89.06%;男女有显著性差异(P<0.01)。在3332例患者中,有417例ENA谱阳性,抗nRNP/Sm阳性102例,抗Sm阳性21例,抗SS-A阳性230例,抗SS-B阳性66例,抗Scl-70阳性20例,抗Jo-1阳性6例,抗ds-DNA阳性106例。结论临床就诊患者中ANA阳性率在不同性别、年龄组间存在差异,ANA阳性荧光模型呈现多样化;与ENA谱联合检测有助于疾病的诊断。

Antinuclear Antibodies and ENA Spectrum Joint Test Value of Clinical Applications

Objective Explore antinuelear antibodies （ANA） and ENA spectrum in medical patients in positive distribution trend. Methods The ANA detection by adopting indirect immunofluorescence, ENA detection by dot method, adopt SPSS11.0 statistical data analysis. Results 3332 patients rate antinuclear antibodies 33.67%, men of 19.74%, positive rate for women, 38.69% both are statistically significant difference （P〈0.0I）. ANA seropositie patients between different age groups of positive also has difference （Less than or equal to 20 years old group accounted for 28.2%, 21~49 years old group 36.4%, is equal to or greater than 50 years old group 29.6%, P〈0.01）. The ANA positive patients with fluorescence mode analysis, type in nuclear particle 28.8%, nuclear homogeneous type 20.3%, of cell plasma particles 11% and nuclear homogeneous/nuclear particle type 10.6% in the majority. The ANA drops degrees of 1 ： 100 p 20.71%, male women make up 89.06%; is equal to or greater than the 1 ： 320 14.78% male accounted for, women make up 85.22%; is equal to or greater than the male accounted for 10.94% 1 ： 1000, women make up 89.06%; men and women have significant difference （P〈0.01）. In 3332 patients, ENA spectrum have 417 cases positive, resistance to nRNP/Sm antibody positive in 102 cases, resistance to Sm antibody positive in 21 cases, resistance to SS-A antibody positive in 230 cases, resistance to SS-B antibody positive in 66 cases, resistance to Scl-70 antibody positive in 20cases, resistance to Jo-1 antibody positive in 6, resistance to ds-DNA antibody positive in 106 cases. Conclusions Clinical medical patient resistance of the nuclear antibody positive rate, in different gender, age groups, there are differences between, resistance to nuclear antibody positive fluorescence model has diversified; ENA spectrum with joint test help disease diagnosis.