大鼠实验性胃癌的凝集素亲合组织化学研究

本文应用花生凝集素(PNA)对17例正常大鼠胃粘膜以及MNNG诱发的22例大鼠腺胃胃癌和14例异型增生胃粘膜进行了亲合组织化学研究。正常大鼠胃粘膜表面上皮不结合PNA;胃底腺PNA结合阳性率为89.6％;幽门腺PNA结合阳性率是17.6％,弥漫分布于腺上皮胞浆内;从界嵴循胃底腺至胃窦幽门腺的顺序观察,PNA结合反应显示逐渐减弱趋势;胃底腺颈、体和底部细胞的PNA结合反应也显示逐渐减弱,大鼠腺胃幽门部胃癌及异型增生腺体的PNA阳性率比正常胃粘膜的相应部位高,PNA结合反应主要沿癌变腺体的腔膜面分布或定位子细胞核上区。本实验结果表明大鼠正常胃粘膜PNA受体的分布具有一定的规律性,大鼠实验性胃癌模型表现有人类胃癌相似的病理生物学特征。

The Study of Lectin Affinity Histochemistry on Experimental Gastric Garcinomas in Rats

We report the affinity histochemical study on 22 gastric carcinomas in glandular stomach and 14 dysplastic gastric mucosas induced by MNNG and 17 normal gastrio mucosas in Wistar rats by using peanut agglutinin (PNA). The superficial epithelium of normal gastric mucosa in rats didn't bind PNA,the positive rate of fundus gland and pyloric gland were 89. 6% and 17. 6% respectively and the positive reaction was distributed diffusely in cytoplasm. Observing from fundus gland to pyloric gland,as well as,from neck,body te bottom of the glands,the reaction of PNA binding was weakened progressively. The positive rate of PNA binding to gastric carcinomas and dysplastic glands in pyloric mucosa was higher than that of the normal gastric mucosa in the correspon ding regions. The staining of PNA binding was mainly distributed along the luminar surface of the glands or in the upper nuclear region of the cells. The results suggest that the distribution of PNA receptor in normal gastric mucosa of rats has a certain regularity and the experimental gastric carcinoma in rats has the same pathobiological feature as human gastric cancer.