苦味受体激动剂苦精对哮喘气道炎症及重塑的影响

目的 观察苦精对哮喘小鼠α-平滑肌肌动蛋白(α-SMA)、气道上皮下胶原纤维及气道炎症的影响.方法 45只BALB/c小鼠分为3组,每组15只.A组:正常对照;B组:哮喘模型组;C组:哮喘加苦精干预1周.采用免疫组织化学方法观察小鼠肺组织α-SMA的沉积,用马松(Masson)染色方法观察小鼠气道胶原纤维的沉积,苏木素-伊红(HE)染色观察小鼠气道炎性程度,并对其进行计算机图像半定量分析.结果 C组小鼠肺内细支气管壁α-SMA、胶原纤维沉积、气道炎症程度较B组明显减少,图像半定量分析结果与B组比较,差异均有统计学意义(P＜0.05).结论 苦精可以改善哮喘小鼠气道炎症及气道重塑.

Effect of denatonium benzoate on airway inflammation and remodeling in asthmatic mice

Objective To evaluate the effect of denatonium benzoaten on α-smooth muscle actin (α-SMA),subepithelial collagen and airway inflammation in asthmu mice.Methods Forty-five BALB/c mice were divided into 3 groups,normal control group (A group),asthma model group (B group),asthma model+ denatonium benzoaten group(C group);α-SMA detected by using immunohistochemistry,lung sections were stained with Masson to detect subepithelial collagen,HE stain method was used to observe the airway inflammation the images were analyzed with semi-quantitative computer.Results The deposition of α-SMA、subepithelial collagen and inflammation degree in C group was significantly reduced compared with B group,the difference were statistically significant(P＜0.05).Conclusion Denatonium benzoaten can improve airway remodeling in asthmatic mice.