HBV adr亚型基因疫苗诱导小鼠产生特异性体液和细胞免疫应答

目的：构建乙型肝炎病毒（HBV)adr亚型基因疫苗pCMV-S2.S(PS），观察其诱导小鼠所产生的特异性体液和细胞免疫应答。方法：将PS注射于C57BL／6小鼠胫骨前肌内，应用ELISA方法检测不同时间小鼠血清中抗HBs抗体以及采用^51Cr释放实验检测淋巴细胞杀伤功能。结果：注射基因疫苗1周后，血清中抗HBs抗体转阳，4周后抗体达到高峰，并以高水平维持至少8周；第12周时PS诱导小鼠产生了良好的HBV特异的细胞免疫应答（P＜0.05）。结论；所构建的HBV adr亚型基因疫苗PS能够诱导小鼠产生较好的体液和细胞免疫应答。

Cellular and humoral immune response to hepatitis B virus adr subtype gene vaccine in mice

Objective:To construct hepatitis B virus(HBV) adr subtype gene vaccine pCMV S2.S and to observe the specific humoral and cellular responses in C57BL/6 mice after gene immunization. Methods:The HBV gene vaccine was injected into tibial anterior muscles of C57BL/6 mice. ELISA was used to detect the anti HBs antibody in mice sera at various time points after gene immunization;HBsAg specific lymphocyte activity was measured by 51 Chromium release assay. Results:Anti HBs in mice sera was positive 1 week after plasmid injection. The level of antibody peaked 4 weeks later, and high titer was kept for at least 8 weeks; The HBsAg specific cellular response was detected on the twelfth weeks. Conclusion:Strong response of humoral and cellular immunity can be induced by injection of pCMV S2.S in C57BL/6 mice.