| 低密度脂蛋白亚组份的分离及初步应用 |
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| 引用本文： | 汪俊军,张允,庄一义,刘小传.低密度脂蛋白亚组份的分离及初步应用[J].医学研究生学报,1998(3). |
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| 作者姓名： | 汪俊军 张允 庄一义 刘小传 |
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| 作者单位： | 南京军区南京总医院解放军医学检验中心 |
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| 基金项目： | 江苏省应用基础研究课题 |
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| 摘 要： | 目的：探讨低密度脂蛋白（ＬＤＬ）亚组份不同特性在动脉粥样硬化中的作用。方法：采用密度梯度超速离心法分离不同密度ＬＤＬ亚组份；分析冠心病（ＣＨＤ）患者和健康人群ＬＤＬ亚组份分布特征。结果：密度梯度超速离心法直接分离出血清中不同密度的ＬＤＬ亚组份，随着密度逐渐增加，电泳迁移率逐渐增大。ＣＨＤ组总ＬＤＬ１、ＬＤＬ２、ＬＤＬ３、ＬＤＬ４显著增高；ＬＤＬ１～４占总ＬＤＬ比例（％）分别为１４．１±４．１、２３．１±７．５、２８．６±９．２、３４．２±１３．７，而对照组为２１．５±１３．５、２１．８±８．７、２７．４±１０．９、２９．３±１３．６，冠心病患者ＬＤＬ分布向小而重ＬＤＬ亚组份倾移。结论：小而重ＬＤＬ亚组份是冠心病发生的危险因素。本法亦为ＬＤＬ亚组份的特性在动脉粥样硬化中的作用研究提供可靠方法
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| 关 键 词： | 低密度脂蛋白 亚组份 分离 冠心病 |
Isolation of low density lipoprotein subfractions and its application |
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| Wang Junjun,Zhang Yong,Zhuang Yiyi et al.Isolation of low density lipoprotein subfractions and its application[J].Bulletin of Medical Postgraduate,1998(3). |
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| Authors: | Wang Junjun Zhang Yong Zhuang Yiyi |
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| Institution: | Wang Junjun,Zhang Yong,Zhuang Yiyi et al Department of Medical Examination,Jinling Hospital |
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| Abstract: | Objectives: To observe low density lipoprotein (LDL) subfractions associations with risk of coronary artery diseases(CHD). Methods: LDL subfractions were isolated directed from plasma by non equilibrium density gradiant centrifugation, and distribution of LDL subfractions in CHD patients were analyzed. Results:The electrophoretic mobility of LDL subfractions was great along the density increasing; the frequency distributions of LDL subfractions in CHD patients were significantly different from that of control. Conclusions: These data support small, density LDL subfraction as a risk factor for atherosclerosis, and this method is useful for investigation of the role of LDL subfractions in development of atherosclerosis. |
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| Keywords: | LDL Subfractions Isolation Coronary artery diseases |
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