| 子宫内膜体外三维重建的形态学研究 |
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| 引用本文： | 王丽,周剑萍,刘银坤,张炜. 子宫内膜体外三维重建的形态学研究[J]. 生殖医学杂志, 2002, 11(6): 335-338 |
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| 作者姓名： | 王丽 周剑萍 刘银坤 张炜 |
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| 作者单位： | 1. 复旦大学妇产科医院,上海,200011
2. 复旦大学中山医院肝癌研究所,上海,200032 |
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| 基金项目： | 上海市科学技术基金重点资助项目 ( 9942 190 0 1) |
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| 摘 要： | 目的 :探索建立子宫内膜体外三维模型的方法。方法 :将分离的子宫内膜基质细胞和上皮细胞依次种植于生物可降解胶原 -聚乳酸羟基乙酸共聚物杂交聚合网上下两面 ,光镜及扫描电镜观察细胞的生长状况。结果 :接种后第 5天子宫内膜细胞能在聚合网上融合生长 ,第 1 4天细胞融合成片 ,呈复层生长 ,形成三维结构。结论 :生物可降解的杂交聚合网作为支架可建立体外子宫内膜三维模型。
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| 关 键 词： | 胶原 生物可降解 多聚乳酸-羟基乙酸共聚物 子宫内膜细胞 组织工程 |
| 文章编号： | 1004-3845(2002)06-0335-04 |
| 修稿时间： | 2002-06-17 |
Study on three-dimensional reconstruction of endometrium in vitro |
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| WANG Li,ZHOU Jian-ping,LIU Yin-kun ,ZHANG Wei Gynecology , Obstetrics Hospital,Fudan University,Shanghai Liver Cancer Institute,Fudan University,Shanghai. Study on three-dimensional reconstruction of endometrium in vitro[J]. Journal of Reproductive Medicine, 2002, 11(6): 335-338 |
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| Authors: | WANG Li ZHOU Jian-ping LIU Yin-kun ZHANG Wei Gynecology & Obstetrics Hospital Fudan University Shanghai Liver Cancer Institute Fudan University Shanghai |
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| Affiliation: | WANG Li,ZHOU Jian-ping,LIU Yin-kun *,ZHANG Wei Gynecology & Obstetrics Hospital,Fudan University,Shanghai 200011 *Liver Cancer Institute,Fudan University,Shanghai 200032 |
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| Abstract: | Objective: To develop a method for reconstructing a three-dimensional model system of endometrium in vitro. Methods: Isolated endometrial stromal cells and epithelial cells were cultured at upper and lower surface on a biodegradable hybrid collagen-PLGA polymer mesh respectively, and observed by scanning electronic microscope and light microscope. Results: It showed that the endometrial cells adhered and spread well on the surfaces of the collagen sponge of the hybrid mesh after being cultured for 5 days. After 2 weeks, the cells proliferated to become confluence achieving a three dimensional structure. Conclusion: The three-dimensional model of endometrium could be established by using the hybrid polymer mesh as a skeleton in vitro. |
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| Keywords: | Collagen Biodegradable Poly(DL-lactic-co-glycolic acid) Endometrial cell Tissue engineering |
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