| 蓝舌病毒HbC株的增殖和血清学特点 |
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| 引用本文： | 陈晓,严银芳,董长垣,陈保平,涂攀,汪凡军. 蓝舌病毒HbC株的增殖和血清学特点[J]. 武汉大学学报(医学版), 1999, 20(3): 179-180 |
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| 作者姓名： | 陈晓 严银芳 董长垣 陈保平 涂攀 汪凡军 |
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| 作者单位： | 湖北医科大学基础医学院病毒学研究所,武汉430071 |
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| 摘 要： | 研究蓝舌病毒中国湖北分离株（ＢＴＶ ＨｂＣ株）在Ｖｅｒｏ和ＢＨＫ ２１两种细胞上的增殖特点以及与标准病毒株（ＢＴＶ １０）的血清学关系。发现ＢＴＶ ＨｂＣ和ＢＴＶ １０感染Ｖｅｒｏ和ＢＨＫ ２１单层细胞后，２４～３０ｈ病毒滴度可达到最高值。用ＢＴＶ ＨｂＣ免疫家兔，其抗血清在琼脂双扩试验中能与ＢＴＶ １０抗原病毒呈强阳性反应而与轮状病毒、风疹病毒、柯萨奇病毒、流感病毒等呈阴性反应。
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| 关 键 词： | 蓝舌病毒；呼肠病毒科；血清学 |
The Characteristics of the Propagation,Purification and Serology of Bluetongue Virus HbC Strain |
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| Chen Xiao,Yan Yinfang,Dong Changyuan,et al. The Characteristics of the Propagation,Purification and Serology of Bluetongue Virus HbC Strain[J]. Medical Journal of Wuhan University, 1999, 20(3): 179-180 |
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| Authors: | Chen Xiao Yan Yinfang Dong Changyuan et al |
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| Abstract: | The characteristics of propagation and serology of bluetongue virus HbC strain (BTV HbC) are reported in this paper.BTV HbC and BTV 10 were respectively incubated onto the monolayers of Vero and BHK 21 cells.These cultures were incubated at 37 ℃,and distinct CPE of them developed in different time.There was the titer peak of BTV in immunodiffusion tests 28 hours later in incubation.The antiserum of BTV HbC reacted positively to the antigen of BTV 10,but negatively to the antigens of rolavirus,influenza virus,rubella virus,etc. |
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| Keywords: | MeSH bluetongue virus reoviridae serology |
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