腺病毒介导的p53对胆管癌细胞生长的抑制作用

目的 研究Ｐ５３基因对胆癌细胞的作用。方法 将重组体腺病毒Ｐ５３转移到人胆管癌细胞ＱＢＣ９３９,用ＲＴ－ＰＣＲ、克隆形成实验、流式细胞仪、ＤＮＡ片段化分析等方法对Ｐ５３基因的表达、细胞的生长抑制及机制进行分析。结果 用Ａｄ－ＬａｃＺ进行重组体腺病毒转导致率的检测,发现当ＭＯＩ为１００以上时,重组体腺病毒可使９０％以上的培养的人胆管癌ＱＢＣ９３９,细胞被传导,用ＲＴ－ＰＣＲ方法检测,在胆管癌ＱＢＣ９

Inhibitory effects of recombinant adenoviruses p53 transduction on human cholangiocarcinoma cell line

AIM To evaluate the inhibitory effect of p53 on human cholangiocarcinoma cell line. METHODS The effects of recombinant adenoviruses p53 (Ad p53) on the growth of QBC939 cells in vitro were analyzed. RESULTS The QBC939 cell line transducted with recombinant adenoviruses LacZ ( Ad LacZ) at MOI of 100 or greater exhibited more than 90% transduction rate. p53 expression was negative in QBC939 cell but high levels of p53 expressions were observed in QBC939 cell line infected with Ad p53. The growth rates of QBC939 cells by Ad p53 at MOI of 100 inhibited reduced by 19.3%(6 day) or 24.4%(8 day) respectively (MOI of 100), compared with those of the Ad LacZ infected cells. Colony formation in these Ad p53 infected cells greatly decreased versus Ad LacZ infected cells. The Ad p53 transfected cells induced QBC939 cell apoptosis and G1 blockade, which was confirmed by the flow cytometry and DNA Ladder. CONCLUSION The suppression effects mediated by expression of the exogenous p53 on tumor cells result mainly from apoptosis and G1 blockade.