Fetal calf serum inhibits virus genome expression in Madin-Darby canine kidney cells persistently infected with influenza A virus |
| |
| Authors: | Md. Jaber Hossain Isamu Mori Li Dong Beixing Liu Yoshinobu Kimura |
| |
| Affiliation: | (1) Department of Microbiology, Fukui University School of Medicine, Fukui 910-1193, Japan;(2) Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Aichi 480-1196, Japan;(3) Department of Medical Technology, Gifu University of Medical Science, 795-1 Nagamine Ichihiraga, Seki, Gifu 501-3892, Japan |
| |
| Abstract: | A cell line of Madin-Darby canine kidney (MDCK) cells persistently infected with human influenza A virus has been established and designated as MDCK-IVpi cells. Production of progeny virus in MDCK-IVpi cells was suppressed when the cells were incubated in the presence of 10% fetal calf serum (FCS). FCS impaired virus mRNA synthesis in MDCK-IVpi cells, which resulted in a scarcity of virus proteins for virion formation. However, MDCK-IVpi cells well supported the growth of superinfecting heterologous influenza viruses, even in the presence of FCS. A certain fetuin-like substance in FCS might be responsible for the observed inhibition of virus replication. |
| |
| Keywords: | Influenza virus Gene expression Blood serum Virus carrier culture |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
