益生菌对急性坏死性胰腺炎大鼠的保护作用研究

目的观察益生菌对急性坏死性胰腺炎大鼠的保护作用，并对其作用机理进行探讨。方法SD大鼠120只，随机分为假手术组、急性坏死性胰腺炎模型组以及大、小剂量益生菌治疗组。大鼠急性坏死性胰腺炎模型是经胆胰管逆行注射5％牛磺胆酸钠形成，益生菌制剂成分为双歧杆菌、乳杆菌和嗜热链球菌。益生菌经灌胃途径预防给药，于造模前3d起，每天给药1次，连续给药3d。分别于造模后6h、12h取血，测定血清淀粉酶、TNF-α和IL-6水平，观察各组动物48h死亡率。结果益生菌能明显降低急性坏死性胰腺炎大鼠48h死亡率，降低血清淀粉酶、TNF-α和IL-6水平。结论益生菌通过抑制炎症因子的表达。对急性坏死性胰腺炎起到保护作用。

Protective effects of probiotics on acute necrotizing pancreatitis in rats

Objective To observe the therapeutic effect of probiotics on acute necrotizing pancreatitis （ANP） in rats. Methods One hundred and twenty SD rats were randomly divided into sham operation group, ANP group and ANP plus differ- ent doses probiotics treated groups. ANP model was induced by intraductal administration of 5% sodium taurocholate. The mix- true of probiotics consisted of Bifidobacterium longum, Lactobacillus bulgaricus and Streptococcus thermophilus. Probiotics were provided to animals by intragastric administration. All treatment started 3d before model induction, and thereafter administered 1 dose a day for 3d. The mortalities of different groups were determined. Serum amylase,TNF-α and IL-6 were measured after model induction （6h, 12h）. Results Compared with model group,the 48h mortality was reduced obviously by probiotics. The levels of serum amylase,TNF-α and IL-6 significantly reduced in probiotics treated groups. Conclusion Probiotics protects acute necrotizing pancreatitis rats by suppressing expression of inflammatory factor.