“10～23”型脱氧核酶的研究进展

脱氧核酶是利用体外分子进化技术获得的一种具有酶活性的单链DNA分子。迄今为止，利用该技术已筛选出多种具有催化功能的脱氧核酶分子，其中研究最多的是具有RNA切割活性的脱氧核酶，尤其是10～23型脱氧核酶，该酶能催化RNA特定部位的切割反应，从mRNA水平使基因灭活，从而调控蛋白质的表达，在抗肿瘤、抗病毒等基因治疗领域具有广阔的应用前景。

Progress in the research on "10-23"deoxyribozyme

Deoxyribozyme is a single-stranded molecule with enzymatic activity. It was derived from a random sequence DNA pool by in vitro selection. Up to date, a variety of deoxyribozymes have been isolated in vitro that can catalyze different chemical reactions. Of particular interest are deoxyribozymes with RNA-cleaving activity, especially “10-23” DNAzyme. It can cleave any RNA in a sequence-specific manner and inactivate gene at the level of mRNA，then regulate the expression of protein. So it may have a wide application prospect in gene therapy as anti cancer and anti viral agents and so on.