中国汉族肢端恶性黑素瘤的差异蛋白质组学研究

目的：研究恶性黑素瘤与正常皮肤组织的蛋白质表达差异，寻找可能的恶性黑素瘤的诊断标志蛋白和治疗靶点。方法：选取经病理确诊的3例恶性黑素瘤切除标本，提取样本的总蛋白质，运用差异凝胶电泳技术分离蛋白质，DeCyder软件分析蛋白图谱的差异，以基质-辅助激光解吸/电离飞行时间串联质谱(MALDI-TOF/TOF-MS)对差异表达蛋白质进行质谱鉴定。结果：质谱鉴定出差异蛋白质点12个，按功能分为4类，其中上调蛋白质3类，为14-3-3蛋白sigma、角蛋白和serpin B3；下调蛋白质1类，为白蛋白。结论：14-3-3蛋白sigma和serpin B3可能参与恶性黑素瘤的发生和发展，其差异表达提示了可能的恶性黑素瘤的发病机制。

Differential proteomic analysis of malignant melanoma in Chinese Han population

Objective To study the proteomic differences between malignant melanoma tissue and normal skin tissue and to look for the possible marker for diagnosis and therapy target of malignant melanoma.Methods Three samples of malignant melanoma tissues were ascertained by pathologic test.The total protein was extracted,separated with the method of 2D-DIGE;the difference of the proteomics was analyzed with DeCyder software;the differential-expressed protein spots were identified with MALDI-TOF/TOF-MS.Results A total of 12 protein spots were identified and categorized into 4 classes according to the function including 3 kinds of up-regulated proteins(14-3-3 protein sigma,keratin and serpin B3),and 1 kind of down-regulated protein(album).Conclusion 14-3-3 protein sigma and serpin B3 are possible involved in the occurance and development of malignant melanoma.The mechanism of album change in the tissue of malignant melanoma remains unclear.The change of those four proteins suggests the possible pathogenesis of malignant melanoma.