肥胖模型大鼠铁代谢和铁营养状态指标的检测及其意义

目的:研究肥胖大鼠铁代谢和铁营养状态,为防治肥胖性铁营养不良提供理论依据.方法:将27只雄性Wistar大鼠随机分为对照组(n=10)和肥胖组(n=17),分别喂养普通饲料和高脂饲料,12周后眼球取血处 死.全血测定血红蛋白、红细胞数、血细胞压积；血清测定血清铁、总铁蛋白结合力、血清转铁蛋白饱和度、铁调素、转铁蛋白及铁...

Detection of iron metabolism and iron nutritional status indicators in obesity rat model and its significance

Objective To study the iron metabolism and iron nutrition state in obesity rats,and to provide the evidence for the prevention and control of iron malnutrition induced by obesity.Methods 27 male Wistar rats were divided into control group(n=10)and obesity group(n=17),the rats were fed with ordinary feed,high fat diet,respectively for 12 weeks,then they were killed by taking the eye blood.The hemoglobin,red cell count,hematocrit,serum iron,total iron binding capacity,serum transferrin saturation,hepcidin,transferring and ferritin of rats were determined.Results Compared with control group,the red cell count and hematocrit value in obesity group were increased,the hemoglobin content was decreased,in which the red blood cell number compared with control group showed significant differences(P<0.05).The serum iron,iron saturation values in obesity group were lower,the serum total iron binding capacity was increased,including serum iron,the iron saturation value compared with control group showed significant differences(P<0.05).The ferritin,transferrin and hepcidin values in obesity group were reduced.Wherein,the hepcidin and ferritin values compared with control group had significant difference(P<0.05).Conclusion Obesity can lead to iron malnutrition and the changes of iron metabolism indices.The obesity subjects should pay attention to iron deficiency.