γ-干扰素对人眼组织共刺激分子及Fas/FasL分子表达的影响

目的 探讨γ-干扰素对人眼组织中共刺激分子及Fas/FasL分子表达的影响。 方法 正常供体人眼9只用于实验。其中6只眼作视网膜平片，每只眼取12片视网膜平片；分别将视网膜平片放于24孔培养板中，共分为3组，每孔含Dulbecco改良Eagle(DMEM)/F12培养液2 ml，3组γ-干扰素（IFN-γ）浓度分别为0、200、1000 U/ml；37℃培养箱中培养(95%O 2，5%CO 2)24h后取出固定，用免疫组织化学方法检测各视网膜平片上B7-1、B7-2、Fas/FasL分子的表达。同时取另外3只正常人眼制作视网膜平片做为正常对照组，直接用免疫组织化学方法检测平片中上述分子的表达。 结果 正常人视网膜平片中有FasL分子的表达，但无B7-1、B7-2和Fas分子表达；当视网膜平片与IFN-γ体外培养后，视网膜中出现B7-1、B7-2和Fas分子的表达，并且FasL分子表达量增多。 结论 IFN-γ可通过诱导共刺激分子及Fas/FasL分子表达参与免疫反应的发生和诱导细胞凋亡，在T淋巴细胞的激活中起着重要作用。 (中华眼底病杂志, 2006, 22: 117-119)

Effects of γ-interferon on the expression of costimulatory molecules and Fas/FasL in human retina

Objective To investigate the effects of γ-interferon (IFN-γ on the expression of costimulatory molecules B7-1 (CD80) and B7-2 (CD86) and Fas/FasL in human retina. Methods Nine human eyes were obtained from the eye-bank of Zhongshan Ophthalmic Center. Six eyes were used for making retinal wholemounts, and 12 retinal wholemounts from each eye were put into the 24-hole culture board which had 2ml DMEM/F12 culture medium in each hole. The wholemounts were divided into 3 groups whose concentration of IFN-γ was 0, 200, and 1000 U/ml respectively. After cultured in 37℃ culture box (95%O 2，5%CO 2) for 24 hours, the expressions of B7-1 (CD80), B7-2 (CD86), and Fas/FasL on these retinal wholemounts were detected by immunohistochemical method. The retinal wholemounts from 3 healthy people were detected by immunohistochemical method as the control. Results Expression of FasL but not B7-1, B7-2 or Fas was found in the control group, while the expression of B7-1, B7-2 and Fas and increased expression of FasL were found after cultured with IFN-γ. Conclusion IFN-γ may be involved in the occurrence of ocular immune response and induction of apoptosis via the stimulation of expression of costimulatory molecules and Fas/FasL, which plays an important role in the activation of T lymphocytes. (Chin J Ocul Fundus Dis, 2006, 22: 117-119)