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In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme L Extract on human lung cancer cell line NCI-H157
Authors:Xiaoni Liu  Yujie Li  Qing Yang  Ying Chen  Xiaogang Weng  Yiwei Wang  Ning Li  Xiaoxin Zhu
Affiliation:Xiaoni Liu (Beijing YouAn Hospital, Capital Medical University, Beijing 100069; China and Institute of Liver Diseases, Beijing 100069, China); Yujie Li (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China); Qing Yang (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China); Ying Chen (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China); Xiaogang Weng (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China); Yiwei Wang (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China); Ning Li (Beijing YouAn Hospital, Capital Medical University, Beijing 100069; China and Institute of Liver Diseases, Beijing 100069, China); Xiaoxin Zhu (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China);
Abstract:

Objective

To investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract (ESC) in vitro.

Methods

ESC was first extracted with ethanol, and then washed using a polyamide column with 60% ethanol. ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions. Subsequently, the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined.

Results

The results showed that ESC was rich in isomers of Chamaejasminor, neochamaejasmine and Sikokianin. ESC had significant cytotoxicity against NCI-H157 cells, with an IC50 of approximately 18.50 μg · mL−1. ESC caused significant increase in total apoptotic rate, the activity of caspase 3 and 8, and Fas protein expression (P<0.05).

Conclusion

The inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway.
Keywords:Stellera chamaejasme L   Lung neoplasms   Apoptosis   Antigens   CD95   Lissamine rhodamine B
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