| 载VEGF反义寡核苷酸PLGA纳米粒的研制 |
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| 引用本文： | 徐爱民,刘善奎,李国栋. 载VEGF反义寡核苷酸PLGA纳米粒的研制[J]. 中国介入影像与治疗学, 2006, 3(1): 55-58 |
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| 作者姓名： | 徐爱民 刘善奎 李国栋 |
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| 作者单位： | 1. 第二军医大学东方肝胆外科医院放射科,上海,200438
2. 第二军医大学药学院,上海,200433 |
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| 基金项目： | 国家自然科学基金;上海市科技发展基金 |
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| 摘 要： | 目的制备血管内皮生长因子反义寡核苷酸(VEGF-ASODN)的PLGA纳米粒,并探讨其制备工艺。方法采用复乳化溶剂挥发法制备,并用正交设计法对纳米粒的处方和制备工艺进行优化。结果纳米粒形态圆整,大小均匀,平均粒径150nm,包封率可达72%,含药量0.84%,体外释放缓慢,达到21天。结论VEGF-ASODN的PLGA纳米粒制备工艺简便、重现性好。
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| 关 键 词： | 聚乳酸羟基乙酸共聚物 血管内皮生长因子 反义寡核苷酸 纳米粒 |
| 文章编号： | 1672-8475（2006）01-0055-04 |
| 收稿时间： | 2005-08-06 |
| 修稿时间： | 2005-11-05 |
Preparation of VEGF antisense oligodeoxynucleotide loaded polylactic-co-glycolic acid nanoparticles |
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| XU Ai-min,LIU Shan-kui and LI Guo-dong. Preparation of VEGF antisense oligodeoxynucleotide loaded polylactic-co-glycolic acid nanoparticles[J]. Chinese Journal of Interventional Imaging and Therapy, 2006, 3(1): 55-58 |
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| Authors: | XU Ai-min LIU Shan-kui LI Guo-dong |
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| Affiliation: | 1. Department of Radiology, Eastern Hepatohiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China ; 2. College of Pharmacy, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | Objective To study vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide (VEGF-ASODN) loaded polylactic-co-glycolic acid (PLGA) nanoparticles and its formulation. Methods PLGA nanoparticles were prepared with w/o/w(water/oil/water) double emulsion-solvent evaporation method by orthogonal design. Results The shape of VEGF-ASODN loaded PLGA nanoparticles is roundness, size is well-distributed, the mean diameter is 150 nm, the encapsulation ratio reaches to 72%, the VEGF-ASODN loading in the nanoparticles is 0.84%, and the release is slow for at least 21 days. Conclusion The preparing method of VEGF-ASODN loaded PLGA nanoparticles is simple, and repeatability is fine. |
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| Keywords: | Polylactic-co-glycolic acid Vascular endothelial growth factor Antisense oligodeoxynuclieotide Nanoparticle |
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