Characterization of a MH2 mutant lacking the v-myc oncogene |
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| Authors: | P Martin C Henry F Denhez P Amouyel C Bechade G Calothy B Debuire D Stehelin S Saule |
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| Institution: | 1. INSERM Unite 186, Institut Pasteur, 1 Rue Calmette, 5,9019 Lille Cedex, France;2. IRCL INSERM Unite 124, Place de Verdun, 59045 Lille Cedex, France;3. Institut Curie-Biologie, Bat. 110, 91405 Orsay, France;1. Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland;2. Empa, Swiss Federal Laboratories for Materials Science and Technology, 9014 St. Gallen, Switzerland;3. Columbia University Department of Systems Biology, New York, NY 10032, USA;4. Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland;5. Department of Molecular Mechanisms of Disease, University of Zurich, 8057 Zurich, Switzerland;6. Faculty of Science, University of Zurich, Zurich, Switzerland;1. School of Energy, Materials and Chemical Engineering, Hefei University, Hefei, 230601, China;2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Science, Beijing, 100190, China;3. State Key Laboratory of Fine Chemicals, Carbon Research Laboratory, School of Chemical Engineering, Dalian University of Technology, Dalian, 116024, China;1. Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA;2. Collaborative Protein Technology Resource, Office of Science and Technology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA;1. Department of Molecular Machines and Signaling, Max Planck Institute of Biochemistry, Martinsried 82152, Germany;2. Department of Structural Biology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA;3. Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany;1. Novartis Institutes for BioMedical Research, Novartis Campus, CH-4056 Basel, Switzerland;2. Department of Biology, ETH Zürich, CH-8093 Zürich, Switzerland |
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| Abstract: | We have previously reported that a virus, MH2-PA200, lacking the ability to transform quail embryo cells, could be isolated from wild type (wt) MH2 stocks passaged on chicken neuroretina cells. We report here the molecular cloning and extensive characterization of this MH2-PA200 provirus. Molecularly cloned MH2-PA200 DNA was found to stimulate the growth of neuroretina cells by transfection assays and our results indicate that this recombinant virus was derived from the RAV-1 helper virus, in which v-mil and a small part of v-myc of MH2 were acquired at the expense of helper (delta gag-pol-delta env) sequences. In order to assess the precise boundary between the myc and env genes we determined the nucleotide sequence of the junction fragment and showed that 11 of 13 nucleotides of the env gene were identical to the myc sequence at the recombination point. The nucleotide sequence of the myc-env junction fragment of another similar and independently generated MH2 mutant showed similarly 9 nucleotides of homology between the env and myc sequences at the recombination point that took place at another site, suggesting that a homologous recombination occurred between MH2 and RAV-1 viruses to generate MH2-PA200 and similar mutants. |
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