| HPLC测定不同产地广东紫珠中3种苯乙醇苷的含量 |
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| 引用本文： | 蔡灏,孙秀漫,欧阳彩君,张蕾,谢智勇,廖琼峰. HPLC测定不同产地广东紫珠中3种苯乙醇苷的含量[J]. 中国实验方剂学杂志, 2013, 19(10): 63-65 |
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| 作者姓名： | 蔡灏 孙秀漫 欧阳彩君 张蕾 谢智勇 廖琼峰 |
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| 作者单位： | 1. 广州中医药大学中药学院,广州,510006
2. 中山大学药学院,广州,510006 |
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| 基金项目： | 国家自然科学基金项目(81173564) |
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| 摘 要： | 目的:采用RP-HPLC-DAD测定不同产地广东紫珠的叶与茎枝中金石蚕苷、毛蕊花糖苷及连翘酯苷B的含量.方法:采用Phenomenex Luna C18色谱柱(4.6 mm ×250 mm,5μm),柱温30℃,流动相乙腈-0.1％乙酸水(20:80),流速1.0mL·min-1,检测波长330 nm.结果:金石蚕苷、毛蕊花糖苷及连翘酯苷B依次在9.96 ～ 498,20.8-1004,10.1～505mg·L-1与峰面积呈良好线性关系；三者平均加样回收率分别为101.3％ (RSD 0.59％),98.7％ (RSD 1.1％),104.2％(RSD 0.71％).不同产地广东紫珠的叶与茎枝中金石蚕苷、毛蕊花糖苷及连翘酯苷B的质量分数范围分别为0.076％ ～ 1.87％,0％～3.19％,0.102％ ～ 1.59％.结论:建立的方法准确、快速、重复性好,可用于同时测定广东紫珠中金石蚕苷、毛蕊花糖苷及连翘酯苷B的含量,为广东紫珠的质量控制提供实验依据.
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| 关 键 词： | 广东紫珠 苯乙醇苷 连翘酯苷B 金石蚕苷 毛蕊花糖苷 含量测定 |
| 收稿时间： | 2012-12-06 |
Content Determination of Three Kinds of Benzene Glycosides in Different Parts of Callicarpa kwangtungensis from Different Areas by HPLC |
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| CAI Hao,SUN Xiu-man,OUYANG Cai-jun,ZHANG Lei,XIE Zhi-yong and LIAO Qiong-feng. Content Determination of Three Kinds of Benzene Glycosides in Different Parts of Callicarpa kwangtungensis from Different Areas by HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(10): 63-65 |
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| Authors: | CAI Hao SUN Xiu-man OUYANG Cai-jun ZHANG Lei XIE Zhi-yong LIAO Qiong-feng |
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| Affiliation: | School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China;School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | Callicarpa kwangtungensis benzene glycosides forsythiaside B poliumoside verbascoside content determination |
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