| HPLC同时测定大花红景天提取物中没食子酸、红景天苷、酪醇、对香豆酸的含量 |
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| 引用本文： | 林夏,胡军华,崔培超,秦建平,仲艳,杨绪芳,李家春,毕宇安,萧伟.HPLC同时测定大花红景天提取物中没食子酸、红景天苷、酪醇、对香豆酸的含量[J].中国实验方剂学杂志,2013,19(10):102-105. |
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| 作者姓名： | 林夏 胡军华 崔培超 秦建平 仲艳 杨绪芳 李家春 毕宇安 萧伟 |
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| 作者单位： | 江苏康缘药业股份有限公司,江苏连云港222001;中药制药过程新技术国家重点实验室,江苏连云港222001 |
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| 基金项目： | 国家科技部"重大新药创制"项目(2011ZX09401-097) |
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| 摘 要： | 目的:建立同时测定大花红景天提取物中没食子酸、红景天苷、酪醇、对香豆酸4种成分含量的HPLC法.方法:Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.3％冰醋酸溶液梯度洗脱,流速0.7 mL· min-,检测波长275,308nm,柱温40℃.结果:没食子酸、红景天苷、酪醇、对香豆酸分别在0.435 ～ 13.912 mg.L-1(r=1,n=6),22.813～730 mg·L-1(r =0.999 8,n=6),7.819 ～250.200 mg·L-1(r =0.999 8,n=6),1.618～51.760 mg·L-1(r=0.9998,＝6)呈良好的线性关系,加样回收率均符合含量测定要求.结论:该含测方法简便、准确,可以用于大花红景天提取物中4个成分的含量测定.
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| 关 键 词： | 高效液相色谱 红景天提取物 没食子酸 红景天苷 酪醇 对香豆酸 含量测定 |
| 收稿时间： | 2012/10/18 0:00:00 |
Determination of Gallic acid, Salidroside, Tyrosol and p-Coumaric Acid in Rhodiola Extract by HPLC |
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| LIN Xi,HU Jun-hu,CUI Pei-chao,QIN Jian-ping,ZHONG Yan,YANG Xu-fang,LI Jia-chun,BI Yu-an and XIAO Wei.Determination of Gallic acid, Salidroside, Tyrosol and p-Coumaric Acid in Rhodiola Extract by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2013,19(10):102-105. |
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| Authors: | LIN Xi HU Jun-hu CUI Pei-chao QIN Jian-ping ZHONG Yan YANG Xu-fang LI Jia-chun BI Yu-an and XIAO Wei |
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| Institution: | Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jangsu Kanion Pharmaceutical CO., LTD, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China |
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| Abstract: | |
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| Keywords: | HPLC Rhodiola extracts gallic acid salidroside tyrosol and p-coumaric acid content determination |
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