几个道地产区茅苍术指纹图谱及苍术素含量测定研究

目的:结合茅苍术主要成分苍术素的含量测定与指纹图谱,比较不同产地药材化学成分的差异性,为科学评价不同产地茅苍术药材的质量提供依据.方法:采用HPLC对各产地茅苍术药材进行分析,采用“中药色谱指纹图谱相似度评价系统”软件比较指纹图谱的相似度,并用SPSS 19.0对13批样品进行聚类分析.采用药典方法测定苍术素含量.结果:建立了茅苍术药材指纹图谱检测方法,不同产地茅苍术药材指纹图谱有较大相似性.但苍术素的含量有较大的差异.聚类分析结果表明道地产区与主产区茅苍术药材存在一定差异,但差别不是很大.结论:所建立的HPLC指纹图谱具有良好的精密度、重复性和稳定性,结合苍术素的含量,为茅苍术质量评价的提供依据.

Study on the Fingerprints of Atractylodes lancea from Different Habitats and the Determination of Atractylodin by HPLC

Objective: To study the HPLC fingerprints and establish a sensitive and specific method for the quality control of Atractylodes lancea from different habitats. Method: All 13 samples of A.lancea from different habitats were determined by RP-HPLC. Use Chromatographic Fingerprint Similarity Evaluation software to analyze data and evaluate HPLC fingerprint established. Result: The simple and specific method with good repeatability was established. There was different content of each component contained in habitat samples produced in individual area. The content of atractylodin had greater difference. Conclusion: The HPLC fingerprint can be used for the quality evaluation of A. lancea.