氟烷和七氟醚对缺血再灌注心肌功能和氧自由基的影响

目的：研究１５肺泡最小浓度（ＭＡＣ）的氟烷和七氟醚对缺血再灌注心肌功能和氧自由基的影响。方法：应用离体大鼠心脏Ｌａｎｇｅｎｄｏｒｆ逆行灌注模型研究１５ＭＡＣ的氟烷、七氟醚对心肌缺血前后心功能的影响，测定缺血前、缺血１０ｍｉｎ、复灌３０ｍｉｎ３个不同时间的心肌超氧化物歧化酶（ＳＯＤ）活性和丙二醛（ＭＤＡ）含量。结果：七氟醚不同程度地抑制心肌收缩功能。缺血１０ｍｉｎ时，七氟醚组ＳＯＤ酶活性明显下降，ＭＤＡ含量显著升高。缺血２５ｍｉｎ复灌３０ｍｉｎ后，二药均能促进心肌功能和ＳＯＤ酶活性恢复，抑制ＭＤＡ生成，其中七氟醚的作用较为明显。结论：二药对缺血再灌注心肌具有一定的保护作用，七氟醚优于氟烷。

Effects of halothane and sevoflurane on myocardial function and oxygen-derived free radicals during ischemia and reperfusion

AIM:To study the effects of 1.5 mininum alveolar concentration (MAC) halothane and sevoflurane on myocardial function and oxygen-derived free radicals during ischemia and reperfusion.METHODS:The model of Langendorff retro-perfused isolated rat heart was used to investigate the effects of halothane and sevoflurane on heart rate (HR),left ventricular end-diastolic pressure, left ventricular diastolic pressure, d p /d t , -d p /d t and coronary flow (CF) before and after ischemia, and the myocardial SOD activity and Malondialdehyde (MDA) content were detemined before and after ischemia 10 minutes,and reperfusion 30 min. RESULTS:In normal isolated rat hearts, CF was increased significantly by sevoflurane, myocardial contractile function were depressed differently by both halothane and sevoflurane. If ischemia held for 10 minutes, SOD activity would significantly decrease and MDA content would significantly increase in sevoflurane grouop. At the end of reperfusion,both of the anesthetics improved the recovery of myocardial function and SOD activity, and depressed the production of MDA,especially sevoflurane. CONCLUSION:Both of the anesthetics could protect myocardium against ischemia and reperfusion injury, especially sevoflurane.