γ射线诱发培养淋巴细胞TCR基因突变的剂量-效应关系

目的　探讨γ射线诱发培养淋巴细胞T细胞受体 (TCR)基因突变的剂量 效应关系。方法　以不同剂量 (0～ 4 0Gy)的γ射线照射新鲜分离的健康成人外周血淋巴细胞 ,植物血凝素脉冲式刺激 2h后 ,用白细胞介素- 2培养 7d ,用单克隆抗体直接免疫荧光标记流式细胞术检测TCR基因突变频率 (TCRMF)。应用SAS统计软件包编写的程序进行辐射剂量 效应曲线的拟合和筛选。结果　培养 7d后 ,TCRMF (10 -4)随照射剂量D (Gy)的增加而增高 ,最佳拟合曲线为二次多项式模型 ,其方程式可描述为 :TCRMF =2 . 74+ 6. 0 5D + 7 60D2 (F =3 62. 3 7 16,P <0 . 0 1,经校正的R2 =0 . 9999)。结论　TCR基因突变作为一种有潜力的生物剂量计可能用于近期辐射照射生物剂量的估算。

The dose-response relationship of T-cell receptor gene mutation induced by gamma-ray in cultured lymphocytes

Objective To investigate the dose-response relationship of T-cell receptor(TCR)gene mutation induced by gamma-ray in cultured lymphocytes.Method Freshly isolated peripheral lymphocytes from healthy adult donors were irradiated with gamma-ray in doses ranging from 0 Gy to 4.0 Cy and cultured with interleukin-2 for 7 days after phytohemagglutinin pulse stimulation for 2 h.The mutant frequencies of TCR gene(TCR MF)were detected by flow cytometry with direct immunofluorescence.Radiation dose-response curves were fitted and an optimal mathematical model for it was selected with programs programmed with Statistical Analysis System(SAS)soft package.Result TCR MF(unit is 10 -4 )increased dose D(in Gy)dependently after cultured for 7 days.Data were found to be fitted optimal by a quadratic polynomial dose-response model,which could be described by the formula TCR MF=2.74+6.05D+7.60D 2(F=36 237.16,P<0.01,adjusted R 2=0.999 9).Conclusion It is suggested that TCR gene mutation,which served as a potential biological dosimeter,may be applied for the estimation of biological dose for recent radiation exposure.