强骨宝对去卵巢大鼠骨量指标与形态计量参数的比较观察

目的观察强骨宝复方制剂干预治疗去卵巢骨质疏松症模型大鼠胫骨钙、镁、磷及羟脯氨酸的含量和骨形态计量参数的变化,并讨论两者变化的相互关系。方法取32只W istar雌性大鼠随机分为4组,按要求行去势手术,然后分别灌胃给药,8周后处死,取大鼠右胫骨烘干测定钙、镁、磷及羟脯氨酸;取左胫骨上段松质骨和中段皮质骨硬组织包埋、切片,图像分析松质骨、皮质骨形态计量参数。结果强骨宝可使去卵巢大鼠胫骨干重,羟脯氨酸、钙、镁与骨干重的比值等骨质量指标明显提高;去卵巢大鼠胫骨松质骨静态参数和动态参数显著增加,吸收参数显著降低;皮质骨静态及动态参数均无显著变化,内膜面骨吸收参数降低。结论强骨宝对去卵巢大鼠骨质疏松症的作用,从骨形态计量参数变化上看能促进骨形成,抑制骨吸收,增加松质骨的密度;从骨质量指标变化来看能促进骨羟脯氨酸等骨胶原有机质的分泌合成,并增加成骨细胞对钙、镁、磷等无机矿物质的吸收和沉积,从而促进新骨的形成,骨质量的增加。因此,骨形态计量参数与骨质量指标两者的变化是统一的。

The Comparatiue Effect of JGB on the Bone Histomorphometry and Bone Quality Parameter in Ovariectomized Rats

ObjectiveTo investigate the changes of the compound of Astragalus membranaceus and Stanozolol(JGB) on the bone histomorphometry and bone quality parameter such as Calcium,Magnesium,Phosphorus,hydroxyproline and bone weight in ovariectomized(OVX) osteoprosis rats,and analyse the mutuality between the changes.Methods32 Wistar female rats,randomly divided into 4 groups were shamly operated or ovariectomized,then all were fed with medicine for two months and then killed.The right tibiae were roasted and weighed to be dissolved by muriatic acid for testing Calcium,Magnesium,Phosphorus,and hydroxyproline.The left proximal and middle tibias were trimed and processed undercalcified sections for cancellous and cortical bone histomorphometric analysis.ResultsThe bone quality parameter such as Calcium,Magnesium,Phosphorus,hydroxyproline and bone weight obviously increased,the static and dynamic parameters of the cancellous bone histomorphometry similarly significantly increased,but the resorption parameters decreased markedly;meantime,the static and dynamic parameters of the cortical bone histomorphometry both had no significant change,while the bone resorption parameter in the endocortical region decreased obviously.ConclusionThe cancellous bone density of OVX rats can be evidently improved by increasing bone formation and inhibiting the endocortical bone resorption.The bone quality also be markedly improved by enhancing hydroxyproline secretion and osteoblast resorption to Calcium,Magnesium and Phosphorus and leading to new bone reconstruction.Therefore,the changes of the bone histomorphometry and bone quality parameter in ovariectomized rats have the same consistency.