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高效液相色谱法测定健儿乐颗粒中芍药苷含量
引用本文:张琼英. 高效液相色谱法测定健儿乐颗粒中芍药苷含量[J]. 中国药业, 2010, 19(17): 23-23
作者姓名:张琼英
作者单位:重庆医科大学附属第二医院,重庆,400010
摘    要:目的测定健儿乐颗粒中芍药苷的含量。方法采用高效液相色谱法,色谱柱为C18柱(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(14∶86),检测波长230nm。结果芍药苷进样量在0.1768~1.7680μg范围内与峰面积线性关系良好,加样回收率为98.60%,RSD为0.48%(n=6)。结论所用方法快速、准确,可作为健儿乐颗粒的质量控制方法。

关 键 词:芍药苷  健儿乐颗粒  高效液相色谱法  含量

Determination of Paeoniflorin Content in Jian'erle Granules by HPLC
Zhang Qiongying. Determination of Paeoniflorin Content in Jian'erle Granules by HPLC[J]. China Pharmaceuticals, 2010, 19(17): 23-23
Authors:Zhang Qiongying
Affiliation:Zhang Qiongying (Second Affiliated Hospital,Chongqing Medical University,Chongqing,China 400010)
Abstract:Objective To determine the content of paeoniflorin in Jian'erle Granules.Methods HPLC method was adopted and the C18 column(250 mm×4.6 mm,5 μm) was used.The mobile phase was acetonitrile-0.1%phosphoric acid(14 ∶86).The detection wavelength was at 230 nm.Results The calibration curve of paeoniflorin was linear in the range of 0.176 8-1.768 0 μg(r=0.999 9),the average recovery rate was 98.60%,RSD=0.48%(n=6).Conclusion The method is quick and accurate,which can be used for the quality control of Jian'erle Granules.
Keywords:paeoniflorin  Jian'erle Granules  HPLC  content
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