特征荧光谱在早期膀胱癌诊断中的应用

目的 寻找紫外激光诱发膀胱癌组织荧光特征光谱并探讨其在膀胱癌早期诊断中的应用价值。方法以血卟啉单甲醚作为光敏剂,全固态紫外激光作为激发光源（波长355nm）,检测膀胱癌组织特征荧光光谱。并对其特性进行分析研究。结果 膀胱癌组织自发荧光在445～490nm处存在双峰状组织特征蜂,经光敏剂孵育后癌组织药物荧光谱除出现组织峰外还在628．65nm处出现特征药物峰。结论 紫外激光诱发膀胱癌组织药物荧光光谱性质稳定,强度佳,特征药物峰处于可见光范围,肉眼易识别,有望提高早期膀胱癌诊断的阳性率。

Early diagnosis of bladder cancer by special fluorescence spectrum: an in vitro experiment

OBJECTIVE: To investigate the value of special fluorescence spectrum in the early diagnosis of bladder cancer. METHODS: Hematoporphyrin monomethyl ether (HMME) of the concentrations of 10, 100, and 200 microg/ml, as photosensitizer, was added into the sample vessels of bladder cancer tissue to be incubated for 1 - 3 h. A detecting system consisting of solid state ultraviolet laser with the wavelength of 355 nm was used to measure the fluorescence spectrum. RESULTS: The laser-induced auto-fluorescence (LIAF) of the bladder cancer tissue had characteristic double peaks at 445 - 490 nm. There was a remarkable characteristic peak, drug peak, located at 628.25 nm in the laser-induced drug-fluorescence (LIDF) of the bladder cancer tissue soaked by HMME. CONCLUSION: Photosentisizer raise the sensitivity and specificity of fluorescence spectrum in diagnosis of bladder cancer. Stable, and with high strength and visible drug peak, the LIDF is may help improve the early diagnosis of bladder cancer.