Sorafenib对前列腺癌DU145细胞的抑制作用的研究

目的观察索拉非尼（Sorafenib）对雄激素非依赖性前列腺癌Du145细胞的抑制作用。方法用不同浓度Sorafenib处理前列腺癌DU145细胞24、48和72h后，MTT法检测Sorafenib对DUl45细胞的抑制作用，流式细胞仪检测细胞凋亡变化，Westernblot检测不同浓度Sorafenib处理72h后DU145细胞内ERK和Bcl-2的表达。结果Sorafenib能显著抑制DU145细胞的体外生长，呈时间与剂量依赖性。DUl45细胞凋亡率随着Sorafenib剂量的增加而增大，具有良好的量效关系（P〈0．01）；Sorafenib处理DU145细胞72h后，ERK和Bcl-2蛋白的表达明显下调（P〈0．01）。结论Sorafenib抑制DU145细胞增殖、诱导细胞凋亡，可显著抑制雄激素非依赖性前列腺癌细胞的体外生长。

Inhibitory action of Sorafenib on the androgen independent prostate cancer cell line DU145 in vitro

Objective To study the inhibitory effects and mechanism of Sorafenib on andro- gen-independent prostate cancer cell line DU145. Methods After being treated with 1-25 μmol Sorafenib for 24-72 h, the inhibiting effece of Sorafenib on the proliferation of DU145 cells was observed by MTT assay, their apoptosis was inspected by flow cytometery （FCM）. The levels of ERK and Bcl-2 in DU145 cells were tested by Western blot. Results Sorafenib could effectively inhibit the growth of androgen independent prost0te cancer cell line DU145 cells in time-dependent and dosedependent manners, induced apoptosis as well （P~0.01）. Sorafenib could decrease the levels of ERK and Bcl-2 in cell line DU145 in a dose-dependent manner （P〈0.01）. Conelasions Sorafenib could significantly inhibit the growth of androgen independent prostate cancer cells DU145 in vitro.