散发性克-雅氏病患者脑脊液中抗炎性细胞因子IL-4水平的检测

目的研究散发性克-雅氏病(sCJD)患者脑脊液中抗炎性细胞因子IL-4的水平,探讨抗炎性细胞因子在CJD疾病过程中的作用。方法病例分为CJD组、其它痴呆组和对照组。采用westernblot法定性分析各组脑脊液中IL-4的表达,用酶联免疫吸附实验检测脑脊液中IL-4的浓度。结果CJD患者脑脊液中IL-4水平明显升高,与其它痴呆组和对照组相比有统计学意义(P<0.05)。结论抗炎性细胞因子IL-4在CJD患者脑脊液中含量增加,在其神经变性过程中可能发挥免疫调节作用,并且有可能成为CJD的生物学标志物。

Detection of anti-inflammatory cytokines interleukin 4 levels in the cerebrospinal fluid of patients with sporadic Creutzfeldt-Jakob disease

Objective To research the levels of anti-inflammatory cytokines interleukin 4 in the cerebrospinal fluid of patients with sporadic Creutzfeldt-Jakob disease and to investigate the potential influence of anti-inflammatory cytokines on the process of (CJD).Methods The group was composed of patients with CJD,patients with other forms of dementia and control subjects.The expression of interleukin 4 in the cerebrospinal fluid of all patients was qualitated using western blot assay and the concentration of the anti-inflammatory cytokines interleukin 4 were determined using an enzyme-linked immunosorbent assay.Results Interleukin 4 levels was significantly elevated in the cerebrospinal fluid of CJD patients.The elevation was significant to other dementia and controls(P<0.05).Conclusions Increase of the anti-inflammatory cytokines interleukin 4 in cerebrospinal fluid of patients with CJD is a new finding. The data of the present study provide a clue toward the possible role of cytokines as immunological modifiers in the neurodegenerative process of CJD,and IL-4 might be a useful biomarker of CJD.