钩端螺旋体疫苗免疫效果玻片凝集试验评价

目的采用TR/Patoc 1玻片凝集试验(SAT)评价钩端螺旋体疫苗免疫效果及应有价值。方法采用TR/Patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化,并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20 d后,玻凝试验抗体阳性率高达90%以上,与标准的显微镜凝集试验比较差异无统计学意义,玻片凝集试验检测的抗体持续时间较短,90 d后阳性率已大幅度降低,表明SAT检测的抗体是早期抗体。结论TR/Patoc 1玻片凝集试验操作简易,可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

Evaluation on slide agglutination assay for immune efficacy vaccination for leptospirosis

ObjectiveTo study the efficacy of the TR/Patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.MethodsUsing TR/Patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test(MAT).ResultsBoth basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in TR/Patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and MAT,the antibody titer was greatly lower in 90 days after basic immunization.ConclusionThe TR/Patoc 1 slide agg lutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.