Effects of curcumin on invasion and metastasis in the human cervical cancer cells Caski |
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Authors: | Fang Xu Xiaoling Mu Jing Zhao |
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Affiliation: | (1) Department of Emergence and ICU, Chongqing Medical University, Chongqing, 400016, China;(2) Department of Gynecology and Obstetrics, the First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China;(3) Department of Pathophysiology, Chongqing Medical University, Chongqing, 400016, China |
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Abstract: | Objective To explore the effects of curcumin on invasion and metastasis in the human cervical cancer cells Caski. Methods Caski cells were treated with 10, 25, 50μmol/L curcumin for 24, 48, 72 h. Proliferation of Caski cells was measured with MTT assay. When treated with 50μmol/L curcumin for 72 h, the expressions of MMP-2, MT1-MMP and NF-κB of cells were detected by Western-blot, and invasion and metastasis of Caski cells were evaluated with transwell chamber. Results After being treated with 10μmol/L, 25μmol/L, 50μmol/L curcumin for 24, 48 and 72 h, the proliferation of Caski cells was inhibited in a dose-and time-dependent manner. The expression of MMP-2, MT1-MMP and NF-κB were decreased when being treated with 50μmol/L curcumin for 72 h. After treatment with 50μmol/L curcumin, in invasion assay, the number of cells in curcumin treated group to migrate to filter coated with Matrigel was reduced compared with control group(P<0.05). Meanwhile, in migration assay, the number of cells in curcumin treated group to migrate to filter was also decreased compared with control group (P<0.05). Conclusion Curcumin could affect the invasion and metastasis of the human cervical cancer cells Caski. Inhibiting the expression of MMP-2, MT1-MMP and NF-κB was probably one of its molecular mechanisms. |
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Keywords: | Cervical cancer Curcumin Invasion Metastasis |
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