参草通脉颗粒影响慢性心力衰竭大鼠血栓形成的实验研究

目的]观察参草通脉颗粒对心力衰竭大鼠血栓形成的影响。方法]选取80只大鼠,随机取20只为正常对照组,其余大鼠经冠脉结扎法配合力竭式游泳、节食等方法造成慢性心力衰竭模型,将造模成功的大鼠分为模型组、中药治疗组、西药治疗组,共4组,均以灌胃给药或蒸馏水28 d。利用蛋白免疫印迹法测定各组血栓素B2(TXB2)、降纤酶原抑制物(PAI-Ⅰ)的变化情况及血清脑钠素(BNP)的浓度,用比色法测定高能磷酸盐(ATP)浓度。结果]与正常对照组相比,模型组及各组大鼠TXB2、PAI-Ⅰ及BNP含量显著升高(P0.01),ATP水平显著降低(P0.01);与模型组相比,中药治疗组和西药治疗组TXB2、PAI-Ⅰ及BNP含量明显降低(P0.01),ATP明显升高(P0.01);西药治疗组与中药治疗组血清中TXB2、PAI-Ⅰ明显降低(P0.01),ATP和BNP含量无明显差异(P0.05)。结论]中药复方参草通脉颗粒可显著降低心力衰竭大鼠BNP的含量水平,降低血小板活化作用,减少心力衰竭后血栓的发生,能够改善线粒体能量代谢,改善心功能,抑制或逆转心室重构。

Experimental study on the effect of Shencao Tongmai granule on thrombosis in chronic heart failure rats

This paper is based on the experience gained trough teaching in the DUMETRAC University degree in traditional Chinese medicine (TCM) at Paris XIII University- Leonard de Vinci Medical School) for 18 years and the newly created DUPRAC (University degree in Mind-Body Practice at Paris VI University- Pierre & Marie Curie Medical School]. Both curriculums are dedicated to French doctors. The teaching of TCM to Westerners requires explaining the fundamental concept of Heaven and also the path and the method that bind Man and Heaven. It is also important to have a common approach to explain the main features of TCM: the feeling and the experience, so that to play a major international role in TCM education.