眼底荧光素血管造影在小鼠氧诱导视网膜病变中的应用评价

目的：：在小鼠氧诱导视网膜病变模型( oxygen-induced retinopathy,OIR )中评价眼底荧光素血管造影( fundus fluorescein angiography,FFA)的应用价值。方法：将前期实验证实视网膜病变严重程度有明显差异的两组( B组>A组)各12只新生幼鼠于出生后第7 d置于75％浓度氧环境中,第12 d时返回正常空气环境中饲养。第17 d时将A组和B组的幼鼠均随机分配,分别进行FFA检查或高分子量FITC-Dextran灌注结合视网膜铺片检查,即每种检查方法纳入两组幼鼠各6只,利用图像分析软件对视网膜无灌注区进行定量分析和比较。结果：FFA结合图像分析软件能对视网膜无灌注区进行定量分析,与FITC-Dextran灌注结合视网膜铺片的测量结果有良好的可比性,两种方法得到的结果无统计学差异(P>0.05)。结论：FFA结合图像定量分析在小鼠OIR模型的血管病变评价中具有一定的实用价值。

Evaluation of fundus fluorescein angiography in mice with oxygen-induced retinopathy

AIM:To investigate avasculopathy-evaluating method using fundus fluorescein angiography(FFA)in mice with oxygen-induced retinopathy(OIR).

METHODS:OIR model was induced by exposure of mice from groups A and B(the retinopathy was known to be more severe in group B than that in group A by previous research)to high oxygen(75%)from postnatal day 7(P7)to P12(n=12 for each group)and returned to normal environment at P12. On P17, the mice from both groups were randomly assigned to accept FFA or high molecular weight fluorescein isothiocyanate dextran(FITC-Dextran)perfusion combined with stretched preparation of retina(each method involved 6 pups from each group). The retinal non-perfused areas were quantified and compared by using image analysis software.

RESULTS: FFA combined with image analysis software was able to quantify the retinal non-perfused areas, which was comparable to the results analyzed by FITC-Dextran perfusion combined with stretched preparation of retina. No statistical difference was found between the results obtained by the two methods(P>0.05).

CONCLUSION:FFA combined with image quantification analysis can be used in retinalvasculopathy evaluation in mouse OIR model.