树突状细胞诱导的CTLs与CIK及LAK细胞对神经胶质瘤杀伤作用比较

目的 比较树突状细胞（DCs）诱导的细胞毒性T淋巴细胞（CTLs）、细胞因子诱导的杀伤细胞（CIK细胞）和淋巴细胞因子激活的杀伤细胞（LAK细胞）对神经胶质瘤的体外杀伤作用。方法 向外周血单个核细胞分别加入不同细胞因子组合，诱导出DCs、CIK细胞和LAK细胞，DCs负载胶质瘤抗原后激活CTLs，MTT法检测对胶质瘤细胞的体外杀伤效应。结果 CTLs对胶质瘤细胞的杀伤作用明显强于CIK细胞和LAK细胞（F=6．42～8．26，q=8．02～10．18，P〈0．01）；CIK细胞对胶质瘤的杀伤作用明显强于LAK细胞（q=8．43～9．24，P〈0．01）。CIK细胞对K562细胞的杀伤作用明显强于LAK细胞和CTLs（F=5．36～7．69，q=7．23～9．56，P〈0．01），LAK细胞对K562细胞的杀伤作用明显强于CTLs（q=8．83～9．15，P〈0．01）。结论 从人外周血中诱导出的DCs负载胶质瘤抗原后，激活的CTLs在体外对胶质瘤细胞能产生高效而特异的杀伤作用，明显强于CIK细胞和LAK细胞，为临床应用DCs瘤苗奠定基础。

CONTRAST AMONG ANTITUMOR EFFICACY OF LAK, CIK AND CTLs ACTIVATED BY DENDRITIC CELLS

Objective To contrast the antitumor efficacy among LAK,CIK and CTLs activated by dendritic cells in vitro. Methods Peripheral blood monocytes were induced to DCs,CIK and LAK by different kinds of cytokine.After being pulsed in vitro with glioma lysates,DCs were used to induce T lymphocytes into cytotoxic T lymphocytes for glioma cells.The cytotoxicity was assayed by MTT. Results CTLs were stronger than CIKs and LAKs in killing glioma cells(F=6.42-8.26,q=8.02-10.18,P<0.01).CIK was stronger than LAK,as well as LAK and CTLs in killing K562 and glioma cells,respectively(q=8.439.24,P<0.01)(F=5.36-7.69,q=7.23-9.56,P<0.01).LAK killed more K562 cells than CTLs did(q=8.83-9.15,P<0.01). Conclusion The antigen specific CTLs can kill glioma cells effectively and specifically,which might play a great role in clinical therapy.