生脉注射液对心肺复苏兔海马神经元超微结构的影响

[目的 ]探讨生脉注射液在心肺复苏中的应用价值及时机。 [方法 ]健康大白兔 30只 ,随机分为 5组 (每组 6只 ) :A组为常规复苏组 ;B组在常规复苏同时加入生脉注射液 5ml;C组在常规复苏开始 5min后 ,加入生脉注射液 5ml;D组在常规复苏开始 10min后加入生脉注射液 5ml;E组在常规复苏开始 15min后加入生脉注射液 5ml。采用窒息法制成心脏骤停动物模型 ,进行心肺复苏 ,电镜观察大脑海马神经元超微结构的变化。 [结果 ]复苏后各组海马神经元细胞的超微结构均存在不同程度的病理改变 ,但各组之间存在差异 ,A组病变最严重 ,B组病变最轻 ,C组、D组、E组病变依次加重 ,但均比A组轻。 [结论 ]生脉注射液可以减轻复苏兔海马神经元细胞超微结构的病理损害 ,且与应用时机有一定的关系 ,应用越早 ,损害越轻。

The Effect of Shengmai Injection(SMI) on Ultrastructure Changes in Hippocampus Neurons in Rabbit during Cardiopulmonary Resuscitation

ObjectiveTo observe the effects of Shengmai Injection on ultrastructure changes in Hippocampus Neurons in rabbits during Cardiopulmonary Resuscitation(CPR).[Methods]Thirty rabbits were randomly divided into 5 groups with 6 in each group: normal CPR (group A),SMI was added immediately when the normal CPR was performed(group B),SMI was added after the normal CPR was performed for 5 minutes(group C),SMI was added after the normal CPR was performed for 10 minutes(group D),SMI was added after the normal CPR was performed for 15 minutes(group E). A cardiac arrest model was induced by asphyxiation in rabbit, then CPR was performed in these animals, and pathological changes in ultrastructrure in Hippocampus Neurons were examined under electron microscopy.[Results]There were damages in ultrastructrure in Hippocampus Neurons of every group with differences in severity. The severity of damage of group A was the most serious and that of group B was the most mild. The severity of damages in group C, D, E became greater successively.[Conclusion]SMI can reduce the pathological lesion in ultrastructrure in Hippocampus Neurons after CPR ,and it has relationship with the applied juncture. The earlier it is applied, the milder the damage is.