| pSVPoMcat微基因修饰雪旺氏细胞移植对脊髓损伤c-fos、bcl-2基因表达的影响 |
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| 引用本文： | 陈礼刚,高立达,曾凡俊,毛伯镛,周虎田,杨立斌,匡永勤.pSVPoMcat微基因修饰雪旺氏细胞移植对脊髓损伤c-fos、bcl-2基因表达的影响[J].西南国防医药,2001,11(4):238-240. |
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| 作者姓名： | 陈礼刚 高立达 曾凡俊 毛伯镛 周虎田 杨立斌 匡永勤 |
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| 作者单位： | 1. 成都军区总医院神经外科
2. 华西医科大学附一院神经外科 |
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| 摘 要： | 目的探讨pSVPoMcat微基因修饰雪旺氏细胞(SC)移植对脊髓损伤(SCI)后c-fos,bcl-2基因表达的影响.方法用切割法制备大鼠SCI模型,将实验动物分为pSVPoMcat微基因修饰SC组(A组),SC移植组(B组),损伤组(C组),正常对照组(D组),不致伤.用地高辛(DIG)标记的c-fos、bcl-2探针行原位杂交.结果SCI后A、B、C、D四组脊髓c-fos基因表达顺序为C＞B＞A=D;bcl-2基因表达顺序为D=A＞B＞C.结论SCI后C-fos基因表达显著增多,而bcl-2基因表达显著减少,pSVPoMcat微基因修饰SC移植能部分抑制SCI后c-fos基因的表达和促进bcl-2基因的表达.
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| 关 键 词： | 脊髓损伤 c-fos基因 bcl-2基因 |
| 修稿时间： | 2001年2月15日 |
Effects of microgene pSVPoMcat implanted to modify Schwann cell on c-fos and bcl-2 gene expression after spinal cord injury in rats |
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| Chen Ligang,Gao Lida ,Zeng Fanjun,Mao Boyong ,Zhou Futian,Yang Libing,Kuang Yongqin.Effects of microgene pSVPoMcat implanted to modify Schwann cell on c-fos and bcl-2 gene expression after spinal cord injury in rats[J].Medical Journal of National Defending forces in Southwest China,2001,11(4):238-240. |
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| Authors: | Chen Ligang Gao Lida Zeng Fanjun Mao Boyong Zhou Futian Yang Libing Kuang Yongqin |
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| Institution: | Chen Ligang,Gao Lida *,Zeng Fanjun,Mao Boyong *,Zhou Futian,Yang Libing,Kuang YongqinDepartment of Neurosurgery,General Hospital of Chengdu Military Command,Chengdu,610083, * : Department of Neurosurgery,FirstAffiliated Hospital of Medical Center of Sichuan University,Chengdu,610041. |
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| Abstract: | objective: To study the effects of microgene pSVPoMcat implanted to modify Schwann cell (SC) on c - fos and bcl - 2 gene expression after spinal cord injury (SCI) in rats. Method: The animals were divided into four groups: the group of microgene pSVPoMcat implanted to modify SC (group A), SC implanted group (group B), injured group (group C) and uninjured control group (group D) . The levels of expression of c - fos and bcl - 2 genes were determined by situ hybridization after operation 8 hours. Result: The c - fos gene expression increased significantly and bcl - 2 gene expression decreased significantly in group C, as compared to those of group D (p < 0.05) . Although c - fos gene expression increased and bcl - 2 gene espression decreased in group A, the changes were not obvious as in group C (p < 0.01) . Conclusion: The c - fos gene expression could be induced and bcl - 2 gene expression be inhibited following SCI. The microgene pSVPoMcat implanted to SC might play some role in inhibition of c - fos gene expression and promotion of bcl - 2 gene expresion following SCI. |
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| Keywords: | spinal cord injury c - fos gene bcl - 2 gene |
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