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| 昼夜节律基因hPerl和hPer2对食管癌细胞株Eca-109放射敏感性的影响 | |
| 引用本文： | 孙凤飞,朱家媛,刘康,李婷婷,李德智,刘延友,李光明. 昼夜节律基因hPerl和hPer2对食管癌细胞株Eca-109放射敏感性的影响[J]. 航天医学与医学工程, 2011, 24(5): 332-335 |
| 作者姓名： | 孙凤飞 朱家媛 刘康 李婷婷 李德智 刘延友 李光明 |
| 作者单位： | 1. 川北医学院第二临床医院,四川,南充,637000 2. 川北医学院,四川,南充,637000 3. 四川大学华西医学中心基础医学与法医学院,四川,成都,610041 |
| 基金项目： | 四川省南充市科学技术计划项目资助(2010SF10) |
| 摘 要： | 目的 探讨昼夜节律基因hPerl和hPer2对食管癌细胞株(Eca-109)放射敏感性的影响.方法 体外培养食管癌Eca-109细胞株,通过避光和乙酰肉豆蔻佛波酯(PMA)分别诱导昼夜节律基因hPerl和hPer2节律性的表达,用RT-PCR检测出表达高峰和低谷.在hPerl和hPer2表达的高峰和低谷时,分别用6mV... |
| 关 键 词： | 昼夜节律基因 食管癌 Eca-109细胞 凋亡 放射敏感性 |
Effects of Circadian Rhythm Genes hPer1 and hPer2 on Radiosensibility of Eca-109 Cells |
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| SUN Feng-fei,ZHU Jia-yuan,LIU Kang,LI Ting-ting,LI De-zhi,LIU Yan-you,LI Guang-ming. Effects of Circadian Rhythm Genes hPer1 and hPer2 on Radiosensibility of Eca-109 Cells[J]. Space Medicine & Medical Engineering, 2011, 24(5): 332-335 | |
| Authors: | SUN Feng-fei ZHU Jia-yuan LIU Kang LI Ting-ting LI De-zhi LIU Yan-you LI Guang-ming |
| Affiliation: | SUN Feng-fei,ZHU Jia-yuan,LIU Kang,LI Ting-ting,LI De-zhi,LIU Yan-you,LI Guang-ming.The Second Clinical Hospital of North Sichuan Medical College,Nanchong Sichuan 637000,China |
| Abstract: | Objective To investigate the effects of circadian rhythm genes-hPer1 and hPer2 on radiosensibility of esophageal cancer cell lines Eca-109.Methods The circadian rhythm expression of hPer1 of esophageal cancer cell lines,Eca-109,was induced with dark-culture.The circadian rhythm expression of hPer2 of Eca-109 was induced with phorbol 12-myristate 13-acetate(PMA).The expression of hPer1 and hPer2 was detected with PT-PCR.Eca-109 cells were irradiated at peak(PG-hPer1,PG-hPer2) and nadir(NG-hPer1,NG-hPer2) of ... |
| Keywords: | circadian rhythm gene esophageal cancer Eca-109 cell apoptosis radiosensibility |
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