短期苯染毒对大鼠中枢神经系统及血液指标的影响

目的观察大鼠在短期苯染毒后的中枢神经系统症状,了解短期苯染毒大鼠血液指标的异常,并探讨这些指标的变化意义。方法取24只成年雌性SD大鼠,随机分成4组(3个实验组,1个对照组),每组各6只,低、中、高剂量组腹腔注射苯,浓度分别39.05、78.11、234.33 mg/kg,对照组为腹腔注射等剂量花生油。观察苯染毒后的中枢神经系统症状,并于染毒24 h后经大鼠股动脉取血,测定各组WBC、RBC、Hb、PLT等血常规指标及ALT、AST、LDH、ALP、BUN、UA等肝肾功能相关指标。结果低、中剂量组大鼠未见明显中枢神经系统异常,高剂量组大鼠出现较明显的中枢神经系统症状。与对照组相比,低、中剂量组血常规指标及肝肾功能相关指标无显著性差别,但高剂量组中WBC、PLT血常规指标及ALT、AST肝功能指标,有显著性差别(P<0.05);各剂量组间、各剂量组与对照组之间的肾功能指标无差异(P>0.05)。结论大鼠在短时间内接触高剂量的苯以后,会出现一定程度的中枢神经系统症状,血常规及肝功能等指标也有一定程度的异常变化,肾功能暂无明显改变。

Short-term central nervous system symptoms and changes in blood indicators after benzene poisoning in rats

Objective To observe the central nervous system symptoms and alterations in the blood indicators in rats within a short term after benzene poisoning.Method Twenty-four female SD rats were randomized into 4 equal groups to receive intraperitoneal injection of low-,medium-or high-dose benzene(39.05,78.11,and 234.33 mg/kg, respectively) or peanut oil.Blood samples were taken from the rats via the femoral artery 24 h after the injections for routine blood test and liver and kidney function test.Results Intraper...