Chk1/2和Plk1蛋白在子宫内膜癌中的表达

目的 Chk1/2（checkpoint kinase 1、2）和Plk1（polo like kinase 1）是各细胞周期检测点启动DNA损伤修复的主要激酶，本研究检测3种激酶在子宫内膜癌及正常子宫内膜组织中的表达，探讨3种蛋白在两者之间的表达差异、与子宫内膜癌临床病理特征的关系及3种蛋白表达的相关性。方法 应用免疫组化SP法检测44例子宫内膜癌组织和21例正常子宫内膜组织中Chk1、Chk2和Plk1蛋白的表达情况。结果 Chk1、Chk2和Plk1蛋白在子宫内膜癌患者中的阳性率分别为47.7％、75.0％和31.8％，在正常子宫内膜中的阳性率分别为61.9％、61.9％和4.8％；Plk1蛋白在子宫内膜癌组织中的表达显著高于正常子宫内膜组织（P＜0.01），而Chk1、Chk2的表达差异无统计学意义（P>0.05）。Chk1、Chk2和Plk1蛋白的表达在不同年龄、病理类型和临床分期的子宫内膜癌患者中差异无统计学意义（P>0.05）；但Chk1的表达在不同分化程度的子宫内膜癌患者中差异有统计学意义（P<0.01）。Spearman等级相关分析，在44例子宫内膜癌患者中，Chk2与Plk1间的表达呈正相关（r＝0.482，P＝0.001）。结论 Plk1可能成为子宫内膜癌比较理想的治疗靶点，而CHK1/2在子宫内膜癌中表达及意义还有待进一步研究。

Expressions of Chk1/2 and Plk1 Protein in Endometrial Carcinoma

Objective 　Chk1/ 2 (checkpoint kinase 1/ 2) and Plk1 (polo2like kinase 1) play the major role in cell cycle checkpoint block when DNA lesion are emerged , this study was to investigate the expression of these kinases in endomet rial carcinoma and healthy endomet ria , and to explore their relationships with clinical pathology parameters of endomet rial carcinoma and the correlations among three kinases. Methods 　Immunohistochemist ry was applied to detect the expressions of Chk1 , Chk2 and Plk1 in 44 cases of en2 domet rial carcinoma and 21 cases of healthy endomet ria. Results 　The positive percentages of Chk1 , Chk2 and Plk1 were 47. 7 %、75. 0 % and 31. 8 % respectively in endomet rial carcinoma , and were 61. 9 %、 61. 9 % and 4. 8 % respectively in healthy endomet ria ; The expression of plk1 proteins in endomet rial car2 cinoma was higher than that in healthy endomet ria , and the difference of plk1 was significant ( P < 0. 01) , while the expression differences of Chk1 and chk2 were no significant ( P > 0. 05) . The expressions of Chk1 , Chk2 and Plk1 were not associated with the age , pathology and clinical stage of endomet rial carci2 noma patient s ( P > 0. 05) , while Chk1 expressions associated with differentiation of endomet rial carcino2 ma ( P < 0. 05) . In 44 endomet rial carcinoma cases , Chk2 was positively correlated with plk1 ( r = 0. 482 , P = 0. 001) . Conclusion 　Plk1 might be ideal target s for endomet rial carcinoma therapy ,while the expres2 sions and significances of CHK1/ 2 in endomet rial carcinoma need further research.